湖羊miR-29a、miR-125b、miR-487b不同生长时期组织表达谱初步分析

发布时间：2018-11-19 12:01

【摘要】：本研究旨在探究miR-29a、miR-125b、miR-487b在湖羊不同生长时期各组织中的表达情况,以了解它们在湖羊肌肉发育中的作用。通过实时荧光定量PCR检测发现,miR-29a在湖羊不同生长时期中差异表达,并在各组织中有着不同的表达模式,其中在背最长肌中的表达显著高于其他肌肉组织和内脏组织;miR-125b只在胎羊和成年羊心肌和背最长肌中的表达相对容易检测,并且在成年羊中的表达明显低于胎羊。结果表明:mi R-125b在湖羊的生长发育过程中表达量下调;miR-487b在湖羊胎羊的不同组织中都有相应的表达,并且在肌肉组织中的表达明显高于其他组织,而在成年羊的相对应的组织中表达量都呈现下调趋势,且背最长肌、腿肌和胸肌的下调趋势与胎羊相应的组织都有显著差异。
[Abstract]:The purpose of this study was to investigate the expression of miR-29a,miR-125b,miR-487b in various tissues of Hu sheep at different growth stages, and to understand their role in muscle development of Hu sheep. It was found by real-time fluorescence quantitative PCR that the expression of miR-29a in the longissimus dorsi muscle was significantly higher than that in other muscle and visceral tissues. The expression of miR-125b was relatively easy to detect in the myocardium and longissimus dorsi of fetal sheep and adult sheep, and the expression of miR-125b in adult sheep was significantly lower than that in fetal sheep. The results showed that the expression of mi R-125b was down-regulated during the growth and development of Hu sheep. The expression of miR-487b in different tissues of Hu sheep fetal sheep was significantly higher than that in other tissues, while the expression of miR-487b in the corresponding tissues of adult sheep showed a downward trend, and the longissimus dorsi muscle showed a down-regulation trend. The down-regulation of leg muscle and pectoral muscle was significantly different from that of fetal sheep.
【作者单位】：江苏师范大学生命科学学院;江苏师范大学科文学院;睢宁县陇西养羊专业合作社;
【基金】：江苏省科技计划项目(BN2016014、BN2015027) 徐州市科技计划项目(KC15N0013、KC16NX073) 江苏省高等学校大学生创新创业训练计划项目(201510320041Z) 江苏师范大学生科院大创项目(201603003);江苏师范大学校科研基金一般项目(11XLB04)
【分类号】：S826

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