体外法研究酵母发酵饲料对瘤胃发酵参数及瘤胃细菌数量的影响

发布时间：2018-11-19 15:01

【摘要】：本试验利用体外法研究日粮中添加酵母发酵饲料对瘤胃发酵参数及瘤胃细菌数量的影响。对照组只含培养底物,试验组在培养底物基础上添加酵母发酵饲料,采用实时荧光定量PCR(qPCR)方法测定瘤胃细菌总数和6种瘤胃功能细菌的数量,同时测定发酵液的产气量、pH、挥发性脂肪酸(VFA)等体外发酵参数。结果表明:酵母发酵饲料能够显著提高瘤胃细菌总数(P0.05),促进溶纤维丁酸弧菌、埃氏巨型球菌、黄色瘤胃球菌、白色瘤胃球菌和琥珀酸丝状杆菌的生长繁殖(P0.05),显著降低牛链球菌数量(P0.05);与对照组相比,试验组的产气量显著增加(P0.05);添加酵母发酵饲料也显著提升了氨态氮(NH_--3-N)的浓度(P0.05)和菌体蛋白(BCP)浓度(P0.05);试验组乙酸、丁酸及总挥发性脂肪酸(TVFA)含量显著高于对照组(P0.05);添加酵母发酵饲料对pH无显著性影响(P0.05)。
[Abstract]:The effects of yeast fermenting feed on rumen fermentation parameters and rumen bacterial count were studied in vitro. The control group contained only the culture substrate, the experimental group was supplemented with yeast fermenting feed, the total number of rumen bacteria and the number of 6 rumen functional bacteria were measured by real-time fluorescence quantitative PCR (qPCR), and the gas production of fermentation broth was determined at the same time. PH, volatile fatty acid (VFA) in vitro fermentation parameters. The results showed that yeast fermenting feed could significantly increase the total number of bacteria in rumen (P0.05), promote Vibrio fibrinolyticus, Macrococcus Ehrlich, Rumen xanthococcus. The growth and reproduction of Streptococcus albicans and filamentous succinate (P0.05) significantly decreased the number of Streptococcus Bovis (P0.05). Compared with the control group, the gas production in the experimental group was significantly increased (P0.05), and the concentration of ammonia nitrogen (NH_--3-N) and bacterial protein (BCP) (P0.05) were also significantly increased by adding yeast fermenting feed (P0.05). The (TVFA) contents of acetic acid, butyric acid and total volatile fatty acids in the experimental group were significantly higher than those in the control group (P0.05), but the addition of yeast fermentation feed had no significant effect on pH (P0.05).
【作者单位】：内蒙古农业大学兽医学院;农业部动物疾病临床诊疗技术重点实验室;内蒙古农牧业科学院动物营养研究所;
【基金】：国家自然科学基金项目(31260560) 内蒙古自治区科技计划项目(自治区应用技术研究与开发资金计划2016) 现代农业(奶牛)产业技术体系建设专项(CARS-37)
【分类号】：S816

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