马铃薯渣发酵蛋白饲料的研究
发布时间：2018-11-20 11:52
【摘要】：本文针对马铃薯淀粉生产中产生的薯渣不易储存和运输、污染环境、开发利用不充分等问题展开研究。以马铃薯渣为主要原料,采用单一菌种和复合菌种发酵试验筛选出最优菌种;利用小麦秸秆,玉米秸秆等农作物废料作为辅料发酵生产蛋白饲料,对蛋白饲料培养基配方进行优化;并研究了用筛选出的菌种固态发酵马铃薯渣生产蛋白饲料的工艺,最后通过体外离体模拟试验对发酵前后的蛋白饲料营养价值进行分析与对比。研究结果如下:(1)通过单菌发酵试验,比较各个菌种发酵后产物中粗蛋白和真蛋白含量的变化情况;再经过复合菌种发酵试验,比较双菌和三菌组合发酵产物中粗蛋白和真蛋白含量的变化情况。结果表明:双菌发酵产物中粗蛋白含量明显高于单菌发酵,三菌组合略低于双菌组合,故确定复合菌种组合为黑曲霉+产朊假丝酵母,其接种比例为1:1.5,接种量为7.5%。(2)以麸皮、玉米秸秆、玉米芯、小麦秸秆、菜籽秸秆等作为辅料发酵生产蛋白饲料,通过单因素试验和响应面法分析优化,结果表明:选择小麦秸秆和麸皮作为发酵辅料,尿素为无机氮源。确定最佳培养基配方为:最佳复合菌种配比(黑曲霉:产朊假丝酵母)为(1:1.5),接种量为7.5%,尿素添加量为2.5%,KH2PO4的添加量为1.5%,辅料(小麦秸秆:麸皮=1;1)添加量为10%。(3)研究了马铃薯渣发酵蛋白饲料的发酵工艺,结合均匀设计和响应面试验,得出最佳的发酵工艺条件为:培养基水分含量为50%;糖化时间为30min;糖化温度为30℃;发酵时间为42h;发酵温度为30℃;酶添加量为4%。测定结果表明,发酵产品中粗蛋白和真蛋白含量较发酵前有显著提高。(4)通过体外离体模拟试验,可以得出,马铃薯渣发酵蛋白饲料与发酵前相比,干物质、粗蛋白、粗纤维素和粗脂肪的消化率均明显提高,粗纤维和粗蛋白的消化率提高最明显,由此得出,微生物发酵的蛋白饲料可提高饲料的利用率。
[Abstract]:In this paper, potato dregs produced in potato starch production are not easy to store and transport, pollute the environment, and develop and utilize them insufficiently. Using potato dregs as the main raw material, the optimum strain was screened by single strain and compound strain fermentation test. Wheat straw, corn straw and other crop wastes were used as auxiliary materials to produce protein feed, and the formula of protein feed medium was optimized. The technology of producing protein feed from potato dregs was studied. Finally, the nutritional value of protein feed before and after fermentation was analyzed and compared by in vitro simulation experiment. The results are as follows: (1) the changes of crude protein and true protein content in the fermentation products of each strain were compared by single strain fermentation test; The changes of crude protein and true protein in the fermentation products of two and three strains were compared. The results showed that the content of crude protein in the fermentation product was significantly higher than that in the single strain fermentation, and the combination of three bacteria was slightly lower than that of the double bacteria combination, so the compound strain combination was determined to be Candida Niger, and the inoculation ratio was 1: 1.5. (2) using wheat bran, corn stalk, corn cob, wheat straw and rapeseed straw as auxiliary materials to produce protein feed, the protein feed was optimized by single factor test and response surface method. The results showed that wheat straw and wheat bran were selected as auxiliary materials and urea as inorganic nitrogen source. The optimum medium formula was determined as follows: the optimum compound strain ratio (Aspergillus Niger: Candida prion) was (1: 1.5), the inoculation amount was 7.5, the amount of urea was 2.5 and the amount of KH2PO4 was 1.5. Auxiliary materials (wheat straw: wheat bran = 1; The results are as follows: (1) the fermentation process of fermented protein feed from potato dregs was studied. The optimum fermentation conditions were as follows: water content in culture medium was 50, saccharification time was 30 minutes, and the optimum fermentation conditions were as follows: (1) the optimum fermentation conditions were: water content in culture medium was 50, saccharification time was 30 minutes, and response surface test was carried out. The saccharification temperature is 30 鈩，
本文编号：2344836
[Abstract]:In this paper, potato dregs produced in potato starch production are not easy to store and transport, pollute the environment, and develop and utilize them insufficiently. Using potato dregs as the main raw material, the optimum strain was screened by single strain and compound strain fermentation test. Wheat straw, corn straw and other crop wastes were used as auxiliary materials to produce protein feed, and the formula of protein feed medium was optimized. The technology of producing protein feed from potato dregs was studied. Finally, the nutritional value of protein feed before and after fermentation was analyzed and compared by in vitro simulation experiment. The results are as follows: (1) the changes of crude protein and true protein content in the fermentation products of each strain were compared by single strain fermentation test; The changes of crude protein and true protein in the fermentation products of two and three strains were compared. The results showed that the content of crude protein in the fermentation product was significantly higher than that in the single strain fermentation, and the combination of three bacteria was slightly lower than that of the double bacteria combination, so the compound strain combination was determined to be Candida Niger, and the inoculation ratio was 1: 1.5. (2) using wheat bran, corn stalk, corn cob, wheat straw and rapeseed straw as auxiliary materials to produce protein feed, the protein feed was optimized by single factor test and response surface method. The results showed that wheat straw and wheat bran were selected as auxiliary materials and urea as inorganic nitrogen source. The optimum medium formula was determined as follows: the optimum compound strain ratio (Aspergillus Niger: Candida prion) was (1: 1.5), the inoculation amount was 7.5, the amount of urea was 2.5 and the amount of KH2PO4 was 1.5. Auxiliary materials (wheat straw: wheat bran = 1; The results are as follows: (1) the fermentation process of fermented protein feed from potato dregs was studied. The optimum fermentation conditions were as follows: water content in culture medium was 50, saccharification time was 30 minutes, and the optimum fermentation conditions were as follows: (1) the optimum fermentation conditions were: water content in culture medium was 50, saccharification time was 30 minutes, and response surface test was carried out. The saccharification temperature is 30 鈩，
本文编号：2344836
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2344836.html
