饲粮铁水平对肉仔鸡锰吸收及组织中沉积的影响
发布时间：2018-11-21 09:30
【摘要】：本试验旨在研究饲粮铁水平对肉仔鸡锰吸收及组织中沉积的影响及其机制。将336只1日龄商品代罗斯308肉公雏按体重随机分为4个组,每组6个重复,每个重复14只鸡,分别饲喂不加铁基础饲粮(含铁77.7 mg/kg)、以硫酸亚铁(Fe SO4·7H2O)形式添加铁100、250、500 mg/kg饲粮。28日龄进行原位结扎十二指肠灌注8.74 mmol/L锰测定锰吸收率。结果表明:血浆铁和血浆转铁蛋白饱和度均随饲粮铁水平升高而提高(P0.10);胰腺、十二指肠黏膜和胫骨灰铁含量随饲粮铁水平升高而升高(P0.10),锰含量随饲粮铁水平升高而降低(P0.10);3个加铁组十二指肠黏膜二价金属转运蛋白m RNA水平均显著低于对照组(P0.10),添加铁500 mg/kg组膜转铁蛋白m RNA水平显著低于对照组和其他2个加铁组(P0.10);加铁组十二指肠锰吸收率均显著低于对照组(P0.10)。结果提示,饲粮铁可能通过调控十二指肠黏膜二价金属转运载体的表达降低锰的吸收及组织中的沉积。
[Abstract]:The purpose of this experiment was to study the effect of dietary iron level on manganese absorption and tissue deposition in broilers and its mechanism. Three hundred and thirty six 1-day-old broilers were randomly divided into 4 groups according to their body weight, 6 replicates in each group and 14 chickens in each replica. They were fed a basal diet without iron (containing 77.7 mg/kg of iron). 100250500 mg/kg diet supplemented with ferrous sulfate (Fe SO4 7H2O) was used to determine the manganese absorptivity by in situ ligation of duodenal perfusion with 8. 74 mmol/L mn at 28 days of age. The results showed that both plasma iron and plasma transferrin saturation increased with the increase of dietary iron level (P0.10). The contents of gray iron in pancreas, duodenal mucosa and tibia increased with the increase of dietary iron level (P0.10), and the content of manganese decreased with the increase of dietary iron level (P0.10). The level of divalent metal transporter m RNA in duodenal mucosa of the three iron-added groups was significantly lower than that in the control group (P0.10), and the level of membrane transferrin m RNA in the iron-added 500 mg/kg group was significantly lower than that in the control group and the other two iron-added groups (P0.10). The absorption rate of duodenum manganese in iron group was significantly lower than that in control group (P 0.10). The results suggest that dietary iron may reduce manganese absorption and tissue deposition by regulating the expression of bivalent metal transport vectors in duodenal mucosa.
【作者单位】： 河北科技师范学院;中国农业科学院北京畜牧兽医研究所;
【基金】：国家自然科学基金(31272465)
【分类号】：S831.5
，
本文编号：2346568
[Abstract]:The purpose of this experiment was to study the effect of dietary iron level on manganese absorption and tissue deposition in broilers and its mechanism. Three hundred and thirty six 1-day-old broilers were randomly divided into 4 groups according to their body weight, 6 replicates in each group and 14 chickens in each replica. They were fed a basal diet without iron (containing 77.7 mg/kg of iron). 100250500 mg/kg diet supplemented with ferrous sulfate (Fe SO4 7H2O) was used to determine the manganese absorptivity by in situ ligation of duodenal perfusion with 8. 74 mmol/L mn at 28 days of age. The results showed that both plasma iron and plasma transferrin saturation increased with the increase of dietary iron level (P0.10). The contents of gray iron in pancreas, duodenal mucosa and tibia increased with the increase of dietary iron level (P0.10), and the content of manganese decreased with the increase of dietary iron level (P0.10). The level of divalent metal transporter m RNA in duodenal mucosa of the three iron-added groups was significantly lower than that in the control group (P0.10), and the level of membrane transferrin m RNA in the iron-added 500 mg/kg group was significantly lower than that in the control group and the other two iron-added groups (P0.10). The absorption rate of duodenum manganese in iron group was significantly lower than that in control group (P 0.10). The results suggest that dietary iron may reduce manganese absorption and tissue deposition by regulating the expression of bivalent metal transport vectors in duodenal mucosa.
【作者单位】： 河北科技师范学院;中国农业科学院北京畜牧兽医研究所;
【基金】：国家自然科学基金(31272465)
【分类号】：S831.5
，
本文编号：2346568
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