福美双诱导的TD肉鸡生长板中P-STAT3、SOCS3基因时序性表达研究

发布时间：2018-11-21 10:20

【摘要】：[目的]研究p-STAT3与SOCS3在肉鸡TD发生过程中的时序性表达,初步探讨JAK-STAT信号通路与肉鸡TD的相关性。[方法]利用实验室建立的肉鸡TD模型,采用Real-time qPCR、免疫组化技术研究TD发生过程中p-STAT3与SOCS3在转录水平和翻译水平的时序性表达差异。[结果]与对照组相比,饲喂福美双组肉鸡生长板STAT3基因在第1天表达变化不明显,第2、4、6天表达增加,差异极显著(P0.01)。SOCS3基因在第1、2、6天表达增加,第4天表达降低,差异极显著(P0.01)。p-STAT3蛋白在生长板增值区、前肥大区与肥大区均表达。与对照组相比,饲喂福美双组增殖区p-STAT3蛋白在第1、2、4天表达均上调,第1、2天差异极显著(P0.01),第4天差异显著(P0.05),第6天表达下调且差异显著(P0.05)；在前肥大区与肥大区,第1、2、4天均上调表达,第6天表达量下调,差异极显著(P0.01)。SOCS3蛋白在生长板各区也均有表达,与对照组相比,饲喂福美双组增殖区SOCS3在第1、4、6天表达上调,第2天表达下调,其中第2、4天差异极显著(P0.01)：在前肥大区,第1、4、6天表达上调,第2天表达下调,其中第1、4天差异极显著(P0.01),第2天差异显著(P0.05);SOCS3在肥大区第1、2、4、6天均表达上调,其中第1、2、4天差异极显著(P0.01)。[结论]在转录水平STAI3与SOCS3基因表达均呈先上升后下降的趋势,差异表达结果与基因芯片结果相吻合。p-STAT3和SOCS3蛋白在生长板增殖区、前肥大区与肥大区均有表达。上述结果提示,福美双可能通过JAK-STAT信号通路调节软骨细胞的增殖、分化及凋亡。
[Abstract]:[objective] to study the temporal expression of p-STAT3 and SOCS3 in broiler TD, and to explore the correlation between JAK-STAT signaling pathway and TD in broilers. [methods] the Real-time qPCR, immunohistochemical technique was used to study the temporal expression differences between p-STAT3 and SOCS3 at transcription level and translation level in the pathogenesis of TD by using the TD model of broilers established in laboratory. [results] compared with the control group, the expression of STAT3 gene in the growth plate of the control group was not significantly changed on the 1st day, but increased on the 2nd day (P0.01). The expression of SOCS3 gene increased on the 1st day (P0.01). On the 4th day, the expression of p-STAT3 protein decreased significantly (P0.01). The expression of p-STAT3 protein was found in the value-added region, prehypertrophic area and hypertrophic region of the growth plate. Compared with the control group, the expression of p-STAT3 protein in proliferative zone was up-regulated on the 1st day (P0.01) and the 4th day (P0.05) in both groups, and the difference was significant on the 1st day (P0.01) and the 4th day (P0.05). On the 6th day, the expression was down-regulated and the difference was significant (P0.05). In the prehypertrophic and hypertrophic regions, the expression of SOCS3 was up-regulated on the 4th day and down-regulated on the 6th day (P0.01). The expression of SOCS3 protein was also found in the growth plate. Compared with the control group, the expression of SOCS3 in the proliferative region of the two groups was significantly higher than that in the control group (P0.01). The expression was up-regulated on the 6th day and down-regulated on the second day. There was a significant difference on the 2nd day (P0.01): in the premast area, the expression of the 1st motif was up-regulated on the 6th day, and the expression was down-regulated on the second day, and the difference was very significant on the 1st day (P0.01). On the second day, the difference was significant (P0.05). The expression of SOCS3 was up-regulated on the 6th day (P0.01). [conclusion] at the transcriptional level, the expression of STAI3 and SOCS3 genes increased first and then decreased, and the differential expression results were consistent with the results of gene microarray. P-STAT3 and SOCS3 proteins were expressed in proliferative, prehypertrophic and hypertrophic regions of the growth plate. These results suggest that Fumex may regulate the proliferation, differentiation and apoptosis of chondrocytes through JAK-STAT signaling pathway.
【学位授予单位】：山西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：Q78;S831

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