一株引起90日龄三黄鸡髓细胞瘤病ALV-J的分离与鉴定

发布时间：2018-11-24 17:24

【摘要】：广西玉林某养殖公司送检的一批疑似感染禽白血病病毒(Avian leukosis virus,ALV)的90日龄三黄鸡表现为精神沉郁、虚弱、消瘦,至送检时发病率为0.2%,无死亡。通过临床症状观察、剖检变化以及病毒分离后细胞培养上清ALV群特异性抗原p27 ELISA检测和细胞培养物亚群特异性PCR的鉴定,从病鸡组织材料中分离到一株ALV-J并命名为GX16ZS01;进一步对其gp85基因进行克隆、测序,并与其他ALV-J参考株进行基因序列的分析与比较,结果表明分离株与ALV-J原型株HPRS-103核苷酸序列的相似性为92.7%,氨基酸序列相似性为88.6%,与其他ALV-J参考株的核苷酸和氨基酸相似性分别为91.1%~95.5%和87.9%~93.2%,其中与SD07LK1的氨基酸相似性最高,为95.5%;遗传系统进化树分析表明,该分离株与GX10YL22株的亲缘关系最近,与原型株HPRS-103亲缘关系较远,但同属ALV-J的大分支;与原型株HPRS-103的糖基化位点比较,发现分离株多出一个糖基化位点。
[Abstract]:A group of 3-yellow chickens suspected to be infected with avian leukemia virus (Avian leukosis virus,ALV) from a breeding company in Yulin, Guangxi, showed depression, weakness and wasting at 90 days, and the incidence rate was 0.2 and no death was found at the time of examination. By observing clinical symptoms, dissecting changes, detecting ALV group specific antigen p27 ELISA in cell culture supernatant after virus isolation and identifying specific PCR of cell culture subgroup, a strain of ALV-J was isolated from tissue material of sick chicken and named GX16ZS01;. The gp85 gene was further cloned, sequenced, and compared with other ALV-J reference strains. The results showed that the nucleotide sequence similarity between the isolated strain and ALV-J prototype strain HPRS-103 was 92.7%. The amino acid sequence similarity was 88.60.The nucleotide and amino acid similarity with other ALV-J reference strains were 91.5% and 87.9%, respectively. The amino acid similarity with SD07LK1 was the highest (95.5%). Phylogenetic tree analysis showed that the isolate had the closest genetic relationship with the GX10YL22 strain, and was far related to the prototype strain HPRS-103, but belonged to the large branch of ALV-J. Compared with the glycosylation site of HPRS-103, one more glycosylation site was found in the isolated strain.
【作者单位】：广西大学养禽与禽病学研究所;
【基金】：现代农业产业技术体系广西肉鸡产业创新团队建设项目(nycytxgxcxtd-04-20-2) 广西研究生教育创新计划资助项目(T3340098003) 公益性行业(农业)科研专项(201203055)
【分类号】：S858.31

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