猪丹毒杆菌活疫苗的安全性试验

发布时间：2018-11-25 09:53

【摘要】：为检测猪丹毒杆菌疫苗的安全性,试验分为猪丹毒疫苗原液组(A1、A2),猪丹毒疫苗分离组(B1、B2),猪场猪丹毒分离组(C1、C2),阳性参照组(D1、D2),阴性参照组E,共九组,每组菌液2个梯度,每个梯度菌液接种10只小鼠。同时疫苗还注射30~40日龄仔猪30头,观察小鼠、小猪生长、死亡、精神状况、剖检情况。结果显示,与疫苗相关的菌株A、B、C组感染小鼠后,小鼠体重、生长、精神状况、剖检情况与阴性参照组相比,没有显著差异,实验结束没有死亡,而阳性参照组小鼠在感染24小时后,出现松毛,扎堆、不食、皮肤发红、精神颓废、于72小时内全部死亡。疫苗注射小猪后,小猪精神状态、生长与对照组相比没有明显差异。结果表明,本试验用猪丹毒杆菌疫苗批次对小鼠、小猪是安全的。
[Abstract]:In order to test the safety of porcine erysipelas vaccine, the test was divided into four groups: porcine erysipelas vaccine (A1A _ 2), porcine erysipelas vaccine isolation (B1B _ 2), pig farm porcine erysipelas isolation (C _ 1C _ 2), positive reference group (D _ 1D _ 2). Negative reference group E, 9 groups, each group of bacteria liquid 2 gradient, each gradient bacteria solution inoculated 10 mice. At the same time, 30 piglets of 40 days old were injected with the vaccine to observe the growth, death, mental status and autopsy of mice and piglets. The results showed that there was no significant difference in the body weight, growth, mental status, dissection status, and no death between the vaccine-related strain Agna BX C group and the negative control group after the infection, and the results showed that there was no significant difference between the negative control group and the control group. In the positive control group, the mice developed loose hair, pile up, no food, skin redness, mental decadence and died within 72 hours after infection for 24 hours. There was no significant difference in mental state and growth between the pigs and the control group. The results showed that the porcine pigs were safe to mice with porcine erysipelas vaccine batches.
【作者单位】：湖南鑫广安农牧股份有限公司;湖南省畜牧兽医研究所;
【分类号】：S858.28

【相似文献】