牛PPARα、PPARγ基因多态性及其与生长性状相关性分析
发布时间：2018-12-08 11:23
【摘要】：肌内脂肪含量是影响牛肉品质的一个重要因素,它直接影响肉的嫩度和风味。过氧化物酶体增殖激活受体PPARα和PPARγ基因是调控肉牛肌内脂肪沉积的相关基因。牛生长性状与脂肪沉积之间具有相关性,本研究以郏县红牛、鲁西牛、南阳牛、秦川牛、渤海黑牛和青海高原牦牛6个品种共计514头牛为实验材料,利用PCR-RFLP结合DNA池测序等技术,研究了与牛脂肪代谢相关PPARa、PPARy基因的遗传多态性,并对上述多态位点与郏县红牛、鲁西牛、南阳牛和秦川牛的多个生长性状指标进行了关联分析,为进一步阐明相关基因影响牛经济性状的分子机制提供参考依据。本研究主要得到以下结果： 1、牛PPARa基因SNPs检测及其与肉牛生长性状相关性分析 在牛PPARa基因检测到3个突变位点,分别为：第2内含子43334bp处A→G突变,第3内含子24bp处G→A突变,第8外显子326bp处T→C突变。在郏县红牛、南阳牛、鲁西牛和秦川牛四个地方黄牛品种中,PPARα基因43334(A/G)、24(G/A)位点呈现中度多态,326(T/C)位点属于低度多态。关联分析显示：43334(A/G)位点多态性与鲁西牛体斜长、十字部高、腹围显著相关(P0.05)；24(G/A)位点多态性与郏县红牛十字部高、坐骨端宽、腰角宽,鲁西牛体高、体斜长、十字部高、胸围、腹围、体重和重高比,河南地方黄牛坐骨端宽(6月龄)、体重(24月龄)和体斜长(24月龄)指标显著相关(P0.05)；326(T/C)位点多态性与河南地方黄牛体斜长(6月龄)指标显著相关(P0.05)。连锁不平衡分析表明43334(A/G)和24(G/A)位点处于强连锁状态,而43334(A/G)与326(T/C)位点之间、24(G/A)与326(T/C)位点之间均为弱连锁或不连锁。在不同群体中,单倍型分布存在差异。 2、牛PPARγ基因SNPs检测及其与肉牛生长性状相关性分析 在牛PPARγ基因中检测到3个突变位点,分别为：PPARγ基因第2内含子59bp处C→G突变,第7外显子20bp处C→T突变,第7外显子50bp处T→C突变。在郏县红牛、南阳牛、鲁西牛和秦川牛四个地方黄牛品种中,PPARy基因59(C/G)位点呈现中度多态,20(C/T)和50(T/C)位点均属于低度多态。关联分析显示：59(C/G)位点多态性与郏县红牛体重,南阳牛体斜长、腹围、腰角宽、坐骨端宽和十字部高指标显著相关(P0.05)；20(C/T)位点多态性与郏县红牛体高和十字部高指标显著相关(P0.05)；50(T/C)位点多态性与南阳牛腰角宽,秦川牛十字部高、重高比指标显著相关(P0.05)。连锁不平衡分析显示,59(C/G)、20(C/T)和50(T/C)三个位点两两之间均处于弱连锁或不连锁状态。单倍型在不同群体中分布存在差异。
[Abstract]:Intramuscular fat content is an important factor affecting beef quality, which directly affects the tenderness and flavor of meat. Peroxisome proliferation-activated receptors (PPAR 伪 and PPAR 纬) are related genes regulating fat deposition in muscle of beef cattle. There was a correlation between growth traits and fat deposition in cattle. In this study, 514 cattle were used as experimental materials, including Jiaxian Red Bull, Luxi cattle, Nanyang cattle, Qinchuan cattle, Bohai Black cattle and Qinghai Plateau Yak. The genetic polymorphisms of PPARa,PPARy gene associated with fat metabolism in cattle were studied by using PCR-RFLP and DNA pool sequencing techniques. The polymorphic loci were compared with Jiaxian red cattle and Luxi cattle. Correlation analysis of several growth traits between Nanyang cattle and Qinchuan cattle was carried out to provide a reference for further elucidating the molecular mechanism of the influence of related genes on cattle economic traits. The main results of this study were as follows: 1. Three mutation sites were detected in bovine PPARa gene by SNPs detection of bovine PPARa gene and its correlation with growth traits of beef cattle. The results were as follows: a / G mutation at intron 2 43334bp; G A mutation in intron 3 24bp and T C mutation in exon 8 326bp. In Jiaxian Red cattle, Nanyang cattle, Luxi cattle and Qinchuan cattle, the PPAR 伪 gene 43334 (A / G), 24 (G / A) loci were moderately polymorphic, and 326 (T / C) loci were low polymorphic. The correlation analysis showed that the polymorphism of 43334 (Ar / G) locus was significantly correlated with the length of the body, the height of the cross and the abdominal circumference of Luxi cattle (P0.05). The polymorphism of 24 (G / A) locus was associated with the height of cross, the width of ischium, the width of waist angle, the height of body, the oblique length of body, the height of cross, chest circumference, abdominal circumference, weight and height of Jiaxian red cattle, and the ischium width of Henan local cattle (6 months old). Body weight (24 months old) and body oblique length (24 months old) were significantly correlated (P0.05); The polymorphism of 326 (T / C) locus was significantly correlated with the body oblique length (6 months old) of Henan local cattle (P0.05). Linkage disequilibrium analysis showed that the 43334 (Ar / G) and 24 (G / A) loci were strongly linked, while 43334 (Ar / G) and 326 (T / C) loci were in a strong linkage state. 24 (G / A) and 326 (T / C) loci were weakly linked or unlinked. The haplotype distribution was different in different populations. 2. SNPs detection of bovine PPAR 纬 gene and its correlation with growth traits of beef cattle revealed three mutation sites in bovine PPAR 纬 gene, namely, C G mutation at 59bp in intron 2 of PPAR 纬 gene and C T mutation at 20bp in exon 7 of PPAR 纬 gene. T-C mutation at exon 7 50bp. In Jiaxian Red cattle, Nanyang cattle, Luxi cattle and Qinchuan cattle, the PPARy gene 59 (C / G) locus was moderately polymorphic, and the 20 (C / T) and 50 (T / C) loci were all low polymorphic. Correlation analysis showed that the polymorphism of 59 (C / G) locus was significantly correlated with Jiaxian red cattle body weight, lean length, abdominal circumference, waist angle width, ischium width and cross height index (P0.05). The 20 (C / T) polymorphism was significantly correlated with the body height and cross height of Jiaxian Red cattle (P0.05), and the 50 (T / C) polymorphism was significantly correlated with the waist angle width of Nanyang cattle, Qinchuan cattle cross height and weight to height ratio (P0.05). Linkage disequilibrium analysis showed that 59 (C / G), 20 (C / T) and 50 (T / C) were weakly linked or unlinked. Haplotypes have different distribution in different populations.
【学位授予单位】：信阳师范学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823
本文编号：2368264
[Abstract]:Intramuscular fat content is an important factor affecting beef quality, which directly affects the tenderness and flavor of meat. Peroxisome proliferation-activated receptors (PPAR 伪 and PPAR 纬) are related genes regulating fat deposition in muscle of beef cattle. There was a correlation between growth traits and fat deposition in cattle. In this study, 514 cattle were used as experimental materials, including Jiaxian Red Bull, Luxi cattle, Nanyang cattle, Qinchuan cattle, Bohai Black cattle and Qinghai Plateau Yak. The genetic polymorphisms of PPARa,PPARy gene associated with fat metabolism in cattle were studied by using PCR-RFLP and DNA pool sequencing techniques. The polymorphic loci were compared with Jiaxian red cattle and Luxi cattle. Correlation analysis of several growth traits between Nanyang cattle and Qinchuan cattle was carried out to provide a reference for further elucidating the molecular mechanism of the influence of related genes on cattle economic traits. The main results of this study were as follows: 1. Three mutation sites were detected in bovine PPARa gene by SNPs detection of bovine PPARa gene and its correlation with growth traits of beef cattle. The results were as follows: a / G mutation at intron 2 43334bp; G A mutation in intron 3 24bp and T C mutation in exon 8 326bp. In Jiaxian Red cattle, Nanyang cattle, Luxi cattle and Qinchuan cattle, the PPAR 伪 gene 43334 (A / G), 24 (G / A) loci were moderately polymorphic, and 326 (T / C) loci were low polymorphic. The correlation analysis showed that the polymorphism of 43334 (Ar / G) locus was significantly correlated with the length of the body, the height of the cross and the abdominal circumference of Luxi cattle (P0.05). The polymorphism of 24 (G / A) locus was associated with the height of cross, the width of ischium, the width of waist angle, the height of body, the oblique length of body, the height of cross, chest circumference, abdominal circumference, weight and height of Jiaxian red cattle, and the ischium width of Henan local cattle (6 months old). Body weight (24 months old) and body oblique length (24 months old) were significantly correlated (P0.05); The polymorphism of 326 (T / C) locus was significantly correlated with the body oblique length (6 months old) of Henan local cattle (P0.05). Linkage disequilibrium analysis showed that the 43334 (Ar / G) and 24 (G / A) loci were strongly linked, while 43334 (Ar / G) and 326 (T / C) loci were in a strong linkage state. 24 (G / A) and 326 (T / C) loci were weakly linked or unlinked. The haplotype distribution was different in different populations. 2. SNPs detection of bovine PPAR 纬 gene and its correlation with growth traits of beef cattle revealed three mutation sites in bovine PPAR 纬 gene, namely, C G mutation at 59bp in intron 2 of PPAR 纬 gene and C T mutation at 20bp in exon 7 of PPAR 纬 gene. T-C mutation at exon 7 50bp. In Jiaxian Red cattle, Nanyang cattle, Luxi cattle and Qinchuan cattle, the PPARy gene 59 (C / G) locus was moderately polymorphic, and the 20 (C / T) and 50 (T / C) loci were all low polymorphic. Correlation analysis showed that the polymorphism of 59 (C / G) locus was significantly correlated with Jiaxian red cattle body weight, lean length, abdominal circumference, waist angle width, ischium width and cross height index (P0.05). The 20 (C / T) polymorphism was significantly correlated with the body height and cross height of Jiaxian Red cattle (P0.05), and the 50 (T / C) polymorphism was significantly correlated with the waist angle width of Nanyang cattle, Qinchuan cattle cross height and weight to height ratio (P0.05). Linkage disequilibrium analysis showed that 59 (C / G), 20 (C / T) and 50 (T / C) were weakly linked or unlinked. Haplotypes have different distribution in different populations.
【学位授予单位】：信阳师范学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823
【参考文献】
相关期刊论文 前1条
1 孔保华,陶菲,刁新平;中国肉牛产业的现状和发展趋势[J];肉类研究;2002年01期
，本文编号：2368264
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