鸡传染性法氏囊病病毒超强毒江苏分离株的鉴定及其致病特征分析

发布时间：2018-12-11 11:15

【摘要】：对2009年采自江苏南京的疑似鸡传染性法氏囊病(IBD)的病料进行了鸡胚分离纯化,通过琼脂扩散、红细胞凝集、血清中和试验、RT-PCR等试验确定分离毒NJ09是传染性法氏囊病病毒(IBDV)。对其VP2基因序列分析显示:NJ09与美国CAHFS_K669分离株及中国的B-SDRZ、SD10LY01分离株亲缘关系较近,共同聚类于一个超强毒株分枝,说明NJ09是一个IBDV超强毒株(vvIBDV)。致病性测定结果显示:NJ09对鸡胚的半数致死量(ELD_(50))为1065/02 mL,对60日龄SPF鸡的攻毒发病率和致死率分别达100%和60%。免疫原性测定结果显示:NJ09的灭活疫苗有较好的保护效果,血清中和抗体值达156 log2。上述结果表明,NJ09是一株vvIBDV,致病力强,免疫原性好,在后续IBDV疫苗研发中是潜在的高效种毒来源。
[Abstract]:Chicken embryo samples from suspected infectious bursal disease (IBD) of chicken were isolated and purified from Nanjing, Jiangsu Province in 2009. The samples were separated and purified by Agar diffusion, erythrocyte agglutination, serum neutralization test. RT-PCR et al. Determine that the isolated virus NJ09 is infectious bursal disease virus (IBDV). The analysis of VP2 gene sequence showed that NJ09 was closely related to the American CAHFS_K669 isolate and the Chinese B-SDRZCS-SD10LY01 isolate, and was clustered in the branch of a super virulent strain, indicating that NJ09 was a IBDV supervirulent strain (vvIBDV). The results of pathogenicity test showed that the ELD_ (50) of NJ09 to chicken embryos was 1065 / 02 mL, and the morbidity and mortality of attacking virus in 60 day old SPF chickens were 100% and 60%, respectively. The results of immunogenicity test showed that the inactivated vaccine of NJ09 had better protective effect, and the neutralization antibody value of serum reached 156 log2.. These results indicate that NJ09 is a highly virulent and immunogenicity strain of vvIBDV, and it is a potential source of highly efficient seed virus in the subsequent development of IBDV vaccine.
【作者单位】：江苏省农业科学院国家兽用制品研究中心;江苏省动物重要疫病与人兽共患病防控协同创新中心;
【基金】：公益性行业(农业)科研专项(201303046)
【分类号】：S858.31

【相似文献】