饲粮菊糖添加水平对肉仔鸡盲肠、直肠菌群结构及主要菌群数量的影响

发布时间：2018-12-14 14:29

【摘要】：本试验旨在研究饲粮菊糖添加水平对肉仔鸡盲肠和直肠菌群结构和主要菌群数量的影响。采用单因素完全随机化设计,选用300只1日龄爱拔益加肉仔鸡,随机分成5组,每组6个重复,每个重复10只。5组肉仔鸡分别饲喂在基础饲粮中添加0(对照组)、0.5、1.5、2.5和5.0 g/kg菊糖的试验饲粮。试验期为6周。采用PCR-变性梯度凝胶电泳(DGGE)和定量PCR(q PCR)技术对42日龄肉仔鸡盲肠和直肠菌群结构及主要菌群数量进行测定。结果表明:1)饲粮菊糖添加水平对肉仔鸡盲肠和直肠菌群丰富度、盲肠菌群香农指数以及直肠菌群均匀度均无显著影响(P0.05),但显著降低了盲肠菌群均匀度和直肠菌群香农指数(P0.05)。PCRDGGE图谱上部分优势条带序列分析表明,饲粮添加菊糖促进了肉仔鸡盲肠和直肠厚壁菌门(Firmicutes)中产粪甾醇真细菌(Eubacterium coprostanoligenes)、肠单胞球菌(Intestinimonas sp.)和盲肠肠球菌(Enterococcus cecorum)等细菌的增殖。2)饲粮菊糖添加水平显著影响了肉仔鸡盲肠、直肠总细菌和大肠杆菌数量(P0.05)。二次曲线分析显示,当菊糖添加水平分别为2.71和2.66 g/kg时,盲肠总细菌和大肠杆菌数量最少;与对照组相比,饲粮添加1.5、2.5、5.0 g/kg菊糖显著降低了盲肠乳酸菌数量(P0.05),饲粮添加0.5 g/kg菊糖显著增加了直肠乳酸菌数量(P0.05);饲粮添加5.0 g/kg菊糖时肉仔鸡盲肠、直肠双歧杆菌数量及直肠产气荚膜梭菌数量均为最高,且与对照组差异显著(P0.05)。综上,在本试验条件下,有利于改善肉仔鸡肠道菌群结构和促进有益菌增殖的菊糖添加水平为2.5~5.0 g/kg。
[Abstract]:The purpose of this study was to study the effect of dietary inulin supplementation on the structure of cecum and rectum flora and the number of main microflora in broilers. Three hundred 1-day-old Aipai plus broilers were randomly divided into 5 groups, 6 replicates in each group and 10 in each group. 5 groups of broilers were fed with 0 (control group) in a basal diet. The experimental diet of 2.5 and 5.0 g/kg chrysanthemum was used. The trial period was 6 weeks. PCR- denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (q PCR) were used to determine the structure of cecum and rectum flora and the number of main microflora in 42-day-old broilers. The results showed that: 1) the dietary inulin supplementation level had no significant effect on the abundance of cecum and rectum flora, the Shannon index of cecum flora and the uniformity of rectum flora in broilers (P0.05). The results showed that the homogeneity of cecum flora and Shannon index of rectum flora were significantly decreased (P0.05). Dietary addition of chrysanthemum promoted fecal sterol production in cecum and rectum (Firmicutes) of broilers with steroidal eubacteria (Eubacterium coprostanoligenes), Enteromonas (Intestinimonas sp.) The proliferation of bacteria such as (Enterococcus cecorum) and cecum cocci. 2) the amount of total bacteria and Escherichia coli in cecum, rectum and Escherichia coli in broilers were significantly affected by dietary inulin supplementation level (P0.05). Quadric curve analysis showed that when the levels of inulin were 2.71 and 2.66 g/kg, the total bacteria and Escherichia coli in the caecum were the least. Compared with the control group, the amount of cecal lactic acid bacteria was significantly decreased when the diet was supplemented with 2.5 g/kg inulin (P0.05), and the number of lactobacillus in rectum was significantly increased by 0.5 g/kg inulin (P0.05). The number of bifidobacterium rectum and Clostridium perfringens were the highest in the cecum of broilers fed with 5.0 g/kg inulin, and the difference was significant compared with the control group (P0.05). In conclusion, under the experimental conditions, the addition level of inulin to improve the intestinal microflora structure and promote the growth of beneficial bacteria in broilers was 2.5% 5.0 g / kg.
【作者单位】：沈阳农业大学畜牧兽医学院;
【基金】：国家自然科学基金项目(31372328)
【分类号】：S831.5

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