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复合微生物固体发酵豆粕的工艺研究及对仔猪生产性能的影响

发布时间:2018-12-17 10:32
【摘要】:豆粕具有较高的粗蛋白含量,并且氨基酸组成较为均衡,是重要的植物源性蛋白饲料。然而植物源性饲料中抗营养因子的含量较高,如抗原蛋白,酶蛋白抑制剂等,严重影响了动物对营养物质的吸收,导致动物生长性能的降低,限制了植物源性饲料在畜牧业及水产养殖业的广泛应用于。因此,豆粕等植物源性饲料的合理开发利用具有重要的应用价值和现实意义。 本文通过乳酸杆菌和芽孢杆菌混合发酵豆粕,探索了混合菌发酵豆粕对仔猪生产性能的影响,同时研究了混合菌发酵豆粕取代鱼粉饲喂仔猪对于仔猪生产性能的影响。复合微生物固体发酵豆粕的工艺研究实验结果显示,乳酸杆菌和芽孢杆菌混合菌种发酵的最佳固液比例为1:0.8,发酵的最佳比例为乳酸杆菌:芽孢杆菌为7:3。最终获得的最佳发酵条件是:发酵温度35℃,以10%的接种量进行接种,培养时间为3天,培养液pH为5.5。发酵后抗原蛋白含量显著降低,粗蛋白含量略微上升,粗纤维含量有所降低,其它的营养物质成分较发酵前基本没有变化。 发酵豆粕对仔猪生产性能的实验结果显示,实验组3在平均日采食量、平均日增重、料重比方面与两个对照组均有显著差异(p0.05),料重比最低,且具有最低的腹泻率。因此,实验组3具有最好的生产性能。固体发酵豆粕对仔猪代谢水平结果显示,各实验小组血清生化指标虽有一定的变化,但都处于正常的水平范围,,各小组之间仔猪的健康状况并没有显著改变,添加5%发酵的豆粕从一定程度上提高了仔猪的生产性能,是更加优质的动物饲料。 仔猪日粮中固体发酵豆粕替代鱼粉的研究结果显示,三个实验组在平均日增重、平均日采食量和料重比方面差异均不显著(p0.05)。在料重比方面,阳性对照组与实验组3的料重比相同,均高于阴性对照组。腹泻率的实验结果显示,实验组3的腹泻率最低,低于阳性对照组的腹泻率,实验组3具有较好的生产性能,因此添加固体发酵豆粕可以取代鱼粉用于仔猪的饲喂。 因此通过乳酸杆菌和芽孢杆菌的混合发酵,我们成功实现了抗原蛋白含量的显著降低,同时保留了豆粕中原有的营养成分。并且,发酵豆粕可以很好的促进仔猪的生长,提高生产性能,并且可以取代鱼粉用于仔猪的喂养。此外,本实验采用的是固体发酵,发酵生产设备相对简单,成本低,耗能低,且实验过程产生的废水、废气等较少,是环保、经济、有效的发酵方法,为养殖和饲料企业提供了优质的蛋白饲料。
[Abstract]:Soybean meal has high crude protein content and balanced amino acid composition. It is an important vegetable protein feed. However, the content of anti-nutritional factors in plant-derived feed is high, such as antigen protein, enzyme protein inhibitor and so on, which seriously affect the absorption of nutrients in animals and lead to the decrease of growth performance of animals. It limits the wide application of botanical feed in animal husbandry and aquaculture. Therefore, the reasonable development and utilization of vegetable feed such as soybean meal has important application value and practical significance. In this paper, the effects of mixed bacteria fermented soybean meal on the performance of piglets were studied by mixed fermentation of Lactobacillus and Bacillus, and the effects of mixed bacteria fermented soybean meal instead of fish meal on the performance of piglets were studied. The optimum solid-liquid ratio of mixed strains of Lactobacillus and Bacillus was 1: 0.8, and the optimum proportion of Lactobacillus and Bacillus was 7: 3. The optimum fermentation conditions were obtained as follows: the fermentation temperature was 35 鈩

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