不同攻毒剂量对评估H9亚型禽流感疫苗免疫效果的影响

发布时间：2018-12-18 03:51

【摘要】：为了解不同攻毒剂量对评估H9亚型禽流感疫苗免疫效果的影响,本研究选取禽流感病毒(AIV)CK/Hu N/174/08和CK/Hu B/S4196/09株制备成灭活疫苗免疫21日龄SPF鸡,免疫后采血检测血凝抑制(HI)抗体,免疫后3周时分别采用这两株亲本病毒经鼻腔途径攻毒。HI抗体测定结果显示:两株疫苗免疫21 d时,平均HI抗体滴度均可达10 log2以上,表明这两株病毒均具有良好的免疫原性。测定每0.1 mL CK/Hu N/174/08株病毒EID_(50)和CID_(50)分别为10~(-9.25)和10~(-3.88),CK/Hu B/S4196/09株为10~(-8.68)和10~(-5.38)。分别以10~6 EID_(50)、10 CID_(50)及10~2 CID_(50)剂量攻毒,CK/Hu N/174/08株和CK/Hu B/S4196/09株疫苗对亲本病毒株攻击的保护率分别为100%和0、100%和80%与60%和0,表明依据EID_(50)和CID_(50)攻毒,CK/Hu N/174/08株疫苗可以得出免疫保护效果一致的结论,而CK/Hu B/S4196/09株疫苗免疫保护效果不一致。本研究表明,EID_(50)和CID_(50)均可以作为H9亚型禽流感疫苗攻毒剂量的参考依据,但以CID_(50)为依据更可靠;10 CID_(50)是最佳的攻毒剂量。本研究为科学评价H9亚型禽流感疫苗的攻毒保护效果提供了依据,对于研制疫苗时选择种毒株以及临床上选用有效疫苗均具有指导意义。
[Abstract]:In order to understand the effect of different doses of virus attack on evaluating the immune effect of H9 subtype avian influenza vaccine, (AIV) CK/Hu N- / 174 / 08 and CK/Hu B/S4196/09 strain were selected to prepare inactivated vaccine to immunize 21-day-old SPF chicken. The hemagglutination inhibition (HI) antibody was detected by hemagglutination after immunization. After 3 weeks of immunization, the two strains were inoculated by nasal route respectively. The results of HI antibody test showed that the average HI antibody titer of the two vaccines was more than 10 log2 after 21 days of immunization. The results showed that the two strains had good immunogenicity. The EID_ (50) and CID_ (50) were 10 ~ (-9.25) and 10 ~ (-3.88) per 0.1 mL CK/Hu N / 174 / 08 strain, respectively, and the CK/Hu B/S4196/09 strains were 10 ~ (-8.68) and 10 ~ (-5.38), respectively. The doses of 10 EID_ (50), 10 CID_ (50) and 10 CID_ (50) were used respectively. The protective rates of CK/Hu N- / 174 / 08 and CK/Hu B/S4196/09 vaccine against parental strain were 100% and 0 100% and 80% and 60% and 0%, respectively, indicating that the virus was attacked according to EID_ (50) and CID_ (50). CK/Hu N- / 174 / 08 vaccine can reach a conclusion that the immune protection effect is the same, but the CK/Hu B/S4196/09 strain vaccine has not the same immune protection effect. The results showed that both EID_ (50) and CID_ (50) could be used as the reference basis for the dose of H9 subtype avian influenza vaccine, but CID_ (50) was more reliable, and 10 CID_ (50) was the best dose. This study provides the basis for the scientific evaluation of the protective effect of H9 subtype avian influenza vaccine, and has guiding significance for the selection of virus strains and the selection of effective vaccine in clinic.
【作者单位】：中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室/兽医生物技术国家重点开放实验室;
【基金】：现代农业产业技术体系专项资金(CARS-42-G08) 国家重点研发计划(2016YFD0501602) 科技基础性工作专项(2013FY113300-8)
【分类号】：S855.3

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