天祝白牦牛前后盘吸虫组织结构的观察
发布时间：2018-12-20 11:17
【摘要】：从甘肃省天祝县采集白牦牛中寄生的前后盘吸虫经处理后经石蜡切片、HE染色和PAS染色后在显徽镜下观察组织结构。结果表明,前后盘吸虫体壁分为两层,外层为角化的上皮组织,中层为排列密集的环形肌;体腔内主要为生殖系统、消化系统和一些肌束和纤维组织,其中生殖系统包括卵巢、子宫、睾丸,而消化管分布于整个体腔;吸盘明显分为三层,外层、中层和内层,中层细胞较多,呈放射状排列,外层为一层较薄的环形肌,内层为一层较厚的排列整齐的环形肌,还有一层较薄的角化皮肤组织。吸盘的中层所占比例较大,主要是发达的呈放射状排列的肌纤维,靠近内层的地方含有大量的嗜碱性细胞,这些细胞大部分成簇存在,分布在肌纤维之间,也有少量的单个存在,散在分布在肌纤维的中间部位。镜下测量,口吸盘较腹吸盘小,比例大约为1∶1.8。PAS染色结果表明,糖原主要分布在生殖系统中,少量分布于吸盘的肌肉层中。压片结果更有易于观察结构的相对位置。
[Abstract]:The parasites from white yak were collected from Tianzhu County of Gansu Province. After treatment, the tissue structure was observed by paraffin section, HE staining and PAS staining. The results showed that the wall of the anterior and posterior disk fluke was divided into two layers, the outer layer was keratinized epithelium and the middle layer was a dense ring muscle. The main body cavity is the reproductive system, digestive system and some muscle bundles and fibrous tissue, in which the reproductive system includes ovary, uterus, testis, and the digestive tract is distributed throughout the body cavity; The sucker is obviously divided into three layers: outer layer, middle layer and inner layer, with more cells in the middle layer, which are arranged radially. The outer layer is a thin ring muscle, the inner layer is a thicker, neatly arranged ring muscle, and there is a thin layer of keratinized skin tissue. The proportion of the middle layer of the sucker is large, mainly the developed radial arrangement of muscle fibers, the place near the inner layer contains a large number of basophilic cells, most of these cells exist in clusters, distributed between the muscle fibers, There is also a small number of individual, scattered in the middle of the muscle fiber. The results of 1:1.8.PAS staining showed that glycogen was mainly distributed in the reproductive system and a little in the muscle layer of the sucker. The results show that the relative position of the structure is easier to be observed.
【作者单位】： 甘肃农业大学动物医学院;
【基金】：国家自然科学基金项目(31460659) 甘肃省国际科技合作专项(144WCGA169)
【分类号】：S852.735
本文编号：2387919
[Abstract]:The parasites from white yak were collected from Tianzhu County of Gansu Province. After treatment, the tissue structure was observed by paraffin section, HE staining and PAS staining. The results showed that the wall of the anterior and posterior disk fluke was divided into two layers, the outer layer was keratinized epithelium and the middle layer was a dense ring muscle. The main body cavity is the reproductive system, digestive system and some muscle bundles and fibrous tissue, in which the reproductive system includes ovary, uterus, testis, and the digestive tract is distributed throughout the body cavity; The sucker is obviously divided into three layers: outer layer, middle layer and inner layer, with more cells in the middle layer, which are arranged radially. The outer layer is a thin ring muscle, the inner layer is a thicker, neatly arranged ring muscle, and there is a thin layer of keratinized skin tissue. The proportion of the middle layer of the sucker is large, mainly the developed radial arrangement of muscle fibers, the place near the inner layer contains a large number of basophilic cells, most of these cells exist in clusters, distributed between the muscle fibers, There is also a small number of individual, scattered in the middle of the muscle fiber. The results of 1:1.8.PAS staining showed that glycogen was mainly distributed in the reproductive system and a little in the muscle layer of the sucker. The results show that the relative position of the structure is easier to be observed.
【作者单位】： 甘肃农业大学动物医学院;
【基金】：国家自然科学基金项目(31460659) 甘肃省国际科技合作专项(144WCGA169)
【分类号】：S852.735
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1 沈亚萍,王索德,崔名标;45日龄仔猪发生前后盘吸虫童虫病的诊疗报告[J];畜牧与兽医;1990年02期
，本文编号：2387919
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