猪细小病毒感染PK-15细胞后TLRs转录时相的变化

发布时间：2018-12-21 13:42

【摘要】：研究猪细小病毒(Porcine Parvovirus,PPV)感染猪肾传代细胞(PK-15 cells)后Toll样受体(Toll-like receptors,TLRs)表达水平变化情况,为探明PPV感染机制提供理论基础。试验利用荧光定量PCR方法检测PPV感染PK-15细胞后TLR1-10的转录时相变化。结果显示,PPV感染PK-15细胞后,TLR1在PPV感染后3h表达上调,约为对照组细胞的3.1倍,其他时段均低于正常水平;TLR3和TLR7 mRNA表达水平在病毒感染早期均低于正常水平;TLR4和TLR8的mRNA含量均在感染后3、12、36 h表达上调,TLR10分别在在3h和36 h表达上调,其他时段均不同程度的降低;而TLR2和TLR9 mRNA表达水平在感染后24 h开始升高,并于48 h达到峰值,分别为对照水平的10.1倍和26.7倍。研究表明,PPV感染PK-15细胞后能够诱导细胞上TLR2和TLR9在mRNA水平显著上调,PPV感染可能通过TLR2和TLR9受体介导感染。
[Abstract]:To study the changes of Toll like receptor (Toll-like receptors,TLRs) expression in porcine renal passage cells (PK-15 cells) infected with porcine parvovirus (Porcine Parvovirus,PPV) and to provide a theoretical basis for understanding the mechanism of PPV infection. Fluorescence quantitative PCR was used to detect the transcriptional phase changes of TLR1-10 after PPV infection in PK-15 cells. The results showed that after PPV infected PK-15 cells, the expression of TLR1 was increased 3 hours after PPV infection, which was 3.1 times of that of the control cells, and the expression of TLR3 and TLR7 mRNA were lower than the normal level in the early stage of the virus infection, and the expression of TLR3 and TLR7 mRNA were lower than the normal level in the early stage of infection. The expression of mRNA in TLR4 and TLR8 was up-regulated at 3125h, TLR10 was up-regulated at 3h and 36h, and decreased in other stages. However, the expression of TLR2 and TLR9 mRNA began to increase 24 h after infection and reached the peak at 48 h, which were 10.1 times and 26.7 times of the control level, respectively. The results showed that TLR2 and TLR9 were significantly up-regulated in PK-15 cells after PPV infection, and PPV infection might be mediated by TLR2 and TLR9 receptors.
【作者单位】：河南农业大学牧医工程学院;河南省动物性食品安全重点实验室;
【基金】：国家自然科学基金项目(U140410985)
【分类号】：S858.28

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