人工合成小肽对肉鸡新城疫疫苗免疫效果影响的研究
发布时间：2018-12-21 21:01
【摘要】：新城疫防控一直是养鸡业疫病防控的一项重要任务。针对现有新城疫疫苗的出现的效价不够强,持续作用时间不够长等问题,研究和开发一种安全可靠兼生产推广成本低的辅助物质一直是该领域研究的热点。本试验旨在通过选用四种人工合成小肽,在接种新城疫疫苗的同时辅助以饮水的方式给肉鸡补饲,以探索不同人工合成小肽对肉鸡新城疫疫苗免疫效果的影响,以期筛选出效果最佳的人工合成小肽及其最适使用剂量,为人工合成小肽在实际生产中的科学使用提供依据。主要研究内容如下:1.四种人工合成小肽制剂对新城疫疫苗免疫肉鸡的效果研究为研究人工合成小肽对肉鸡血清中鸡新城疫疫苗抗体效价的影响,将900羽1日龄AA肉鸡随机平均分配到空白对照组、新城疫疫苗组、新城疫疫苗+T4-3H4组、新城疫疫苗+T4-3T4组、新城疫疫苗+3H4组和新城疫疫苗+3T4组,每组设3个重复,每个重复50羽。每组鸡分别自由饮用含不同人工合成小肽的饮用水,在免疫后的第0、7、14、21、28和35天分别从每个重复组中随机挑选2只鸡采集血液样品,测定血清中的鸡新城疫疫苗抗体水平。结果表明:以饮水方式给肉鸡饲喂四种人工合成小肽制剂,可以有效提高不同日龄鸡血清新城疫疫苗的抗体水平,其中3H4人工小肽的效果最佳,由此可见,以饮水方式给肉鸡补饲人工合成小肽制剂有助于提高鸡新城疫疫苗的免疫效果。2.人工合成小肽3H4的最适使用浓度研究为探索人工合成小肽3H4的最适使用浓度,将270羽1日龄AA肉鸡随机平均分配到高浓度3H4处理组(2ug/150L)、中浓度3H4处理组(1ug/150L)和低浓度3H4处理组(0.5ug/150L),每组设置3个重复组,每个重复30只鸡。每组鸡分别自由饮用含不同浓度的人工合成小肽(3H4)的饮用水,在免疫后的第0、7、14、21、28和35天分别从每个重复组中随机挑选2只鸡采集血液样品,测定血清中的鸡新城疫疫苗抗体水平。结果表明:饮用水添加中浓度(1ug/150L)人工合成小肽3H4时的鸡新城疫疫苗免疫效果最佳。
[Abstract]:Newcastle disease prevention and control has been an important task of chicken industry epidemic prevention and control. In order to solve the problem that the titer of Newcastle disease vaccine is not strong enough and the duration of action is not long enough, the research and development of a safe and reliable auxiliary substance with low production and popularization cost has been a hot spot in this field. The purpose of this study was to investigate the effect of different synthetic peptides on the immune effect of Newcastle disease vaccine in broilers by selecting four kinds of synthetic peptides and giving them supplementary feeding with drinking water while inoculating Newcastle disease vaccine. In order to screen out the best synthetic small peptide and its optimal dosage, to provide the basis for the scientific use of synthetic small peptide in practical production. The main contents are as follows: 1. Study on the effects of four synthetic small Peptide preparations on Newcastle Disease Vaccine Immunization in Broiler chickens; in order to study the effect of synthetic small peptides on the antibody titer of Newcastle disease vaccine in serum of broilers, 900 1-day-old AA broilers were randomly and equally assigned to the blank control group. Newcastle disease vaccine group, Newcastle disease vaccine T4-3H4 group, Newcastle disease vaccine T4-3T4 group, Newcastle disease vaccine 3H4 group and Newcastle disease vaccine 3T4 group were divided into 3 replicates with 50 replicates per group. The chickens of each group drank freely drinking water containing different synthetic peptides. On the 0th day after immunization, two chickens were randomly selected from each repeated group to collect blood samples, and the antibody level of Newcastle disease vaccine in serum was determined. The results showed that feeding broilers with four synthetic small peptide preparations in drinking water could effectively improve the antibody level of Newcastle disease vaccine in serum of chickens of different days, and the effect of 3H4 artificial small peptide was the best. Supplemental feeding of synthetic small peptide to broilers with drinking water was helpful to improve the immune effect of Newcastle disease vaccine. 2. Study on the optimal concentration of synthetic small Peptide 3H4 in order to explore the optimal concentration of synthetic small Peptide 3H4, 270 AA broilers of 1 day old were randomly assigned to the high concentration 3H4 treatment group (2ug/150L). There were 3 repeats in each group, 30 chickens in each group. The medium concentration 3H4 treatment group (1ug/150L) and the low concentration 3H4 treatment group (0.5ug/150L) were treated with 3 repeats in each group. Drinking water containing different concentrations of synthetic small peptide (3H4) was freely consumed in each group. Two chickens were randomly selected from each repeated group to collect blood samples on the 28th and 35th day after immunization. The antibody level of Newcastle disease vaccine in serum was determined. The results showed that the best immune effect of Newcastle disease vaccine was obtained when drinking water was supplemented with medium concentration (1ug/150L) of small peptide 3H4.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.31
本文编号：2389457
[Abstract]:Newcastle disease prevention and control has been an important task of chicken industry epidemic prevention and control. In order to solve the problem that the titer of Newcastle disease vaccine is not strong enough and the duration of action is not long enough, the research and development of a safe and reliable auxiliary substance with low production and popularization cost has been a hot spot in this field. The purpose of this study was to investigate the effect of different synthetic peptides on the immune effect of Newcastle disease vaccine in broilers by selecting four kinds of synthetic peptides and giving them supplementary feeding with drinking water while inoculating Newcastle disease vaccine. In order to screen out the best synthetic small peptide and its optimal dosage, to provide the basis for the scientific use of synthetic small peptide in practical production. The main contents are as follows: 1. Study on the effects of four synthetic small Peptide preparations on Newcastle Disease Vaccine Immunization in Broiler chickens; in order to study the effect of synthetic small peptides on the antibody titer of Newcastle disease vaccine in serum of broilers, 900 1-day-old AA broilers were randomly and equally assigned to the blank control group. Newcastle disease vaccine group, Newcastle disease vaccine T4-3H4 group, Newcastle disease vaccine T4-3T4 group, Newcastle disease vaccine 3H4 group and Newcastle disease vaccine 3T4 group were divided into 3 replicates with 50 replicates per group. The chickens of each group drank freely drinking water containing different synthetic peptides. On the 0th day after immunization, two chickens were randomly selected from each repeated group to collect blood samples, and the antibody level of Newcastle disease vaccine in serum was determined. The results showed that feeding broilers with four synthetic small peptide preparations in drinking water could effectively improve the antibody level of Newcastle disease vaccine in serum of chickens of different days, and the effect of 3H4 artificial small peptide was the best. Supplemental feeding of synthetic small peptide to broilers with drinking water was helpful to improve the immune effect of Newcastle disease vaccine. 2. Study on the optimal concentration of synthetic small Peptide 3H4 in order to explore the optimal concentration of synthetic small Peptide 3H4, 270 AA broilers of 1 day old were randomly assigned to the high concentration 3H4 treatment group (2ug/150L). There were 3 repeats in each group, 30 chickens in each group. The medium concentration 3H4 treatment group (1ug/150L) and the low concentration 3H4 treatment group (0.5ug/150L) were treated with 3 repeats in each group. Drinking water containing different concentrations of synthetic small peptide (3H4) was freely consumed in each group. Two chickens were randomly selected from each repeated group to collect blood samples on the 28th and 35th day after immunization. The antibody level of Newcastle disease vaccine in serum was determined. The results showed that the best immune effect of Newcastle disease vaccine was obtained when drinking water was supplemented with medium concentration (1ug/150L) of small peptide 3H4.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.31
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