产肠毒素性大肠杆菌侵染猪肠上皮细胞后DLG5基因的表达变化分析
发布时间：2018-12-24 13:34
【摘要】：为了揭示DLG5基因在机体抵抗大肠杆菌刺激过程中发挥的功能,本试验利用产肠毒素性大肠杆菌(F18ab、F18ac和K88ac)侵染猪肠上皮细胞系(IPEC-J2)模拟个体的致病过程,用实时荧光定量检测DLG5基因在感染前后的表达变化情况,在细胞水平上探讨DLG5基因表达水平与产肠毒素性大肠杆菌抗性的关系。结果表明,3种大肠杆菌的菌清和菌体刺激IPEC-J2后,DLG5基因表达水平均发生显著或极显著上调;并且由F18ab和K88ac这2种菌体刺激后DLG5基因表达水平上调的程度要极显著高于F18ac菌体刺激后的表达水平。本研究结果提示,猪DLG5基因表达和大肠杆菌的抗性具有密切关系,可能与其在一定程度上能够促进肠道屏障的结构稳定和功能发挥有关。
[Abstract]:In order to reveal the function of DLG5 gene in resisting the stimulation of Escherichia coli, we used enterotoxigenic Escherichia coli (F18abanF18ac and K88ac) to infect porcine intestinal epithelial cell line (IPEC-J2) to simulate the pathogenicity of the individual. The expression of DLG5 gene before and after infection was detected by real-time fluorescence quantitative analysis. The relationship between the expression level of DLG5 gene and the resistance of enterotoxigenic Escherichia coli was investigated at the cellular level. The results showed that the level of DLG5 gene expression was significantly up-regulated or extremely significantly up-regulated by the three Escherichia coli strains and the IPEC-J2 stimulated by the bacteria. The expression level of DLG5 gene stimulated by F18ab and K88ac was significantly higher than that stimulated by F18ac. The results suggest that the expression of porcine DLG5 gene is closely related to the resistance of Escherichia coli, and it may be related to the structural stability and function of intestinal barrier.
【作者单位】： 扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室;江苏省种猪繁育和健康养殖工程技术研究中心;
【基金】：国家自然科学基金(编号:31372285) 江苏省科技支撑计划(编号BE2014357) 江苏省苏州市科技局攻关项目(编号:SNG201628)
【分类号】：S852.61
，
本文编号：2390687
[Abstract]:In order to reveal the function of DLG5 gene in resisting the stimulation of Escherichia coli, we used enterotoxigenic Escherichia coli (F18abanF18ac and K88ac) to infect porcine intestinal epithelial cell line (IPEC-J2) to simulate the pathogenicity of the individual. The expression of DLG5 gene before and after infection was detected by real-time fluorescence quantitative analysis. The relationship between the expression level of DLG5 gene and the resistance of enterotoxigenic Escherichia coli was investigated at the cellular level. The results showed that the level of DLG5 gene expression was significantly up-regulated or extremely significantly up-regulated by the three Escherichia coli strains and the IPEC-J2 stimulated by the bacteria. The expression level of DLG5 gene stimulated by F18ab and K88ac was significantly higher than that stimulated by F18ac. The results suggest that the expression of porcine DLG5 gene is closely related to the resistance of Escherichia coli, and it may be related to the structural stability and function of intestinal barrier.
【作者单位】： 扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室;江苏省种猪繁育和健康养殖工程技术研究中心;
【基金】：国家自然科学基金(编号:31372285) 江苏省科技支撑计划(编号BE2014357) 江苏省苏州市科技局攻关项目(编号:SNG201628)
【分类号】：S852.61
，
本文编号：2390687
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