腹泻仔猪肠道致病性大肠埃希氏菌和沙门氏菌的分离鉴定与耐药性研究

发布时间：2018-12-25 10:14

【摘要】：为探明仔猪细菌性腹泻肠道致病性大肠埃希氏菌和沙门氏菌流行的血清型、耐药表型及耐药基因型,本试验采集了贵州省5个地(州)市7个规模化养猪场的128份腹泻仔猪肠道样本,并对采集的样本进行了大肠埃希氏菌和沙门氏菌分离与鉴定,通过动物试验鉴定菌株的致病性,利用血清学方法鉴定其血清型,并通过药敏纸片法对主要致病菌进行耐药性研究,采用PCR技术检测各致病菌株耐药相关基因,分析细菌耐药表型和耐药基因型相关性。结果显示,本研究共分离鉴定到78株致病性大肠埃希氏菌与21株沙门氏菌,致病性大肠埃希氏菌血清型以O138、O87为主,沙门氏菌血清型以鼠伤寒沙门氏菌、肠炎沙门氏菌居多;药敏试验结果表明,本试验分离到的78株致病性大肠埃希氏菌对β-内酰胺类药物耐药率达80%以上,对其他种类的抗菌药耐药率均超过40%,分离鉴定的21株沙门氏菌对氨基糖苷类药物耐药率达50%以上,对其他种类的抗菌药耐药率均达20%以上;本试验分离鉴定的致病性大肠埃希氏菌共检出12种耐药相关基因,沙门氏菌共检出10种耐药相关基因,两种细菌耐药基因型与耐药表型符合率均达60%以上,且均为多重耐药。本研究为仔猪腹泻的综合防控提供了理论依据。
[Abstract]:To identify the prevalent serotypes, drug resistance phenotypes and drug resistance genotypes of enteropathogenic Escherichia coli and Salmonella in piglets with bacterial diarrhea. In this experiment, 128 intestinal samples of diarrhea piglets were collected from 7 large-scale pig farms in 5 places (states) of Guizhou Province, and the samples were isolated and identified by Escherichia coli and Salmonella. The pathogenicity of the strain was identified by animal test, serotype was identified by serological method, drug resistance of main pathogenic bacteria was studied by drug sensitive disk method, and drug resistance related genes were detected by PCR technique. To analyze the correlation between drug resistance phenotype and drug resistance genotype of bacteria. The results showed that 78 strains of pathogenic Escherichia coli and 21 strains of salmonella were isolated and identified in this study. The main serotypes of pathogenic Escherichia coli were O138O87, Salmonella typhimurium and Salmonella enteritidis were the main serotypes. The results of drug sensitivity test showed that 78 strains of pathogenic Escherichia coli were more than 80% resistant to 尾 -lactam, and more than 40% to other kinds of antimicrobial agents. The resistant rate of 21 Salmonella strains to aminoglycoside was more than 50% and 20% to other kinds of antimicrobial agents. A total of 12 drug-resistance-related genes were isolated from pathogenic Escherichia coli and 10 drug-resistance-related genes were detected from Salmonella. The coincidence rates of drug resistance genotypes and phenotypes of two bacteria were over 60%, and they were multidrug resistant. This study provides a theoretical basis for comprehensive prevention and control of diarrhea in piglets.
【作者单位】：贵州大学动物科学学院;贵州省动物疫病预防控制中心;水城县农业局;
【基金】：贵州省科学技术厅农业攻关项目“贵州省仔猪腹泻性疫病风险分析及综合防控技术研究”(黔科合NY[2015]3009-1号) “贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队”(黔科合人才团队[2015]4016号) 贵州省农业委员会科技计划项“贵州省2015年重大动物疫病省级定点监测”[2015-01号]
【分类号】：S852.61

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