狐源豚鼠气单胞菌M12的鉴定及致病性分析

发布时间：2018-12-26 17:02

【摘要】：为了鉴别豚鼠气单胞菌M12并研究其致病性,本试验利用管家基因gyrB鉴定菌种,然后采用家兔回肠体外器官培养(IVOC)和腿部肌肉注射两种方式探究该病原菌的致病机制,运用光学显微镜、电子显微镜、扫描电镜等多种手段从微观视角去研究M12的菌体结构、致病过程和感染组织的病理变化。结果显示,M12为两端钝圆的革兰氏阴性短杆菌,在电镜下观察发现该菌除鞭毛外细菌大小为(0.6~1.6)μm×(0.6~0.7)μm,鞭毛长度是菌体长度的2~3倍。该菌与GenBank上豚鼠气单胞菌的相似性高达100.0%。IVOC试验在扫描电镜下观察可以看到M12黏附在肠上皮细胞聚集成团,形成生物膜结构,肠上皮细胞破损;病理切片观察发现M12对家兔肠、肝脏、肺脏、肾脏、肌肉组织都有损伤。本试验结果可为豚鼠气单胞菌致病性的深入研究提供参考依据。
[Abstract]:In order to identify and study the pathogenicity of Aeromonas guinea-pig strain M12, the strain was identified by housekeeping gene gyrB, and then the pathogenicity of the pathogen was investigated by means of (IVOC) culture in vitro and intramuscular injection of leg in rabbit ileum. By means of optical microscope, electron microscope and scanning electron microscope, the mycelium structure, pathogenicity and pathological changes of infected tissue of M12 were studied from the microscopic perspective. The results showed that M12 was a Gram-negative short bacilli with obtuse circles at both ends. The size of the bacteria except flagella was (0.6 ~ 1.6) 渭 m 脳 (0.6 ~ 0. 7) 渭 m, and the length of flagellum was 2 ~ 3 times longer than that of bacteria under electron microscope. The similarity between this strain and Aeromonas guinea-pig on GenBank was as high as that of 100.0%.IVOC test. Under scanning electron microscope, M12 adhered to intestinal epithelial cells to form clusters, forming biofilm structure, and intestinal epithelial cells were damaged. Pathological observation showed that M 12 damaged the intestine, liver, lung, kidney and muscle tissue of rabbits. The results of this study can provide a reference for the further study of the pathogenicity of Aeromonas guinea-pig.
【作者单位】：中国农业科学院特产研究所;军事医学科学院;
【基金】：基金项目:毛皮动物重要传染病快速诊断体系的建立(20130077)
【分类号】：S852.61

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