绿原酸抑制金黄色葡萄球菌机理研究

发布时间：2018-12-30 16:22

【摘要】：为探索绿原酸(CGA)抑制金黄色葡萄球菌增殖的机制,通过检测β-半乳糖苷酶的变化反映细菌细胞膜通透性的变化,凝固酶的活性反映细菌致病力;用Bradford法、DNS法和DNP法分别检测可溶性蛋白质量浓度、还原糖利用能力和丙酮酸质量浓度的变化反映细菌代谢的改变.结果显示CGA处理金黄色葡萄球菌后菌液中β-半乳糖苷酶出现泄漏,且漏出量与CGA质量浓度呈量效关系,无血浆凝固现象,菌液中还原糖质量浓度急剧升高,可溶性蛋白质量浓度降低量超过50%,丙酮酸质量浓度升高5.34%,与对照组相比差异极具统计学意义(p0.01),表明CGA可能通过改变金黄色葡萄球菌细胞膜的通透性,阻碍物质和能量的代谢及细菌蛋白合成而达到抑菌作用.
[Abstract]:In order to explore the mechanism of Lv Yuan (CGA) inhibiting the proliferation of Staphylococcus aureus, the changes of 尾 -galactosidase and coagulase activity were detected to reflect the change of cell membrane permeability and pathogenicity of bacteria. The changes of soluble protein concentration, reducing sugar utilization ability and pyruvate mass concentration were measured by Bradford method, DNS method and DNP method respectively. The results showed that there was leakage of 尾 -galactosidase in the solution of Staphylococcus aureus treated with CGA, and there was a dose-effect relationship between the amount of leakage and the concentration of CGA. There was no plasma coagulation, and the concentration of reducing sugar in the solution increased sharply. The content of soluble protein decreased by more than 50%, the mass concentration of pyruvate increased 5.34%, the difference was statistically significant compared with the control group (p0.01), indicating that CGA may change the permeability of staphylococcus aureus cell membrane. The bacteriostatic effect is achieved by blocking the metabolism of substance and energy and the synthesis of bacterial protein.
【作者单位】：西南大学(荣昌校区)兽医科学研究中心中药创新研究室;
【基金】：国家公益性行业(农业)科技专项经费资助(20130304-05) 西南大学荣昌校区大学生课外科技立项资助(20121020-02)
【分类号】：S853.7

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