长链非编码RNA的调控机制及其在家畜中的预测方法

发布时间：2018-12-30 17:34

【摘要】：长链非编码RNA(long noncoding RNA,lncRNA)是一类转录本长度超过200个核苷酸,不参与蛋白编码的功能性RNA分子,直接以RNA形式在表观遗传水平、转录水平、转录后水平、翻译水平和翻译后水平调控基因的表达,在家畜体内的各种生物学过程中起重要作用。作者对长链非编码RNA的来源、分类和结构特征、实现生物学功能的4种分子调控机制(信号分子、诱饵分子、引导分子、支架分子)、调控基因表达的5个层面及采用高通量RNA测序技术分析测序数据和预测家畜lncRNA的方法进行了综述,为合理地研究家畜的lncRNA提供基础。家畜lncRNA预测方法主要包括深度测序、转录组重建、新转录本预测、lncRNA识别4个部分,在lncRNA识别阶段,作者所在研究团队前期采用将蛋白质编码潜能评估工具CPAT(coding potential assessment tool)和在线的序列编码能力预测工具CPC(coding potential calculator)结合取交集的方法,较好地从预测得到的大量的候选转录本中快速排除具蛋白编码能力的转录本,预测获得68 604条内蒙古绒山羊的皮肤组织lncRNA,丰富了NONCODE数据库中羊lncRNA转录本的基础数据。
[Abstract]:Long strand noncoding RNA (long noncoding RNA,lncRNA) is a class of functional RNA molecules with length of more than 200 nucleotides that are not involved in protein coding and are directly expressed as RNA at the epigenetic level and post-transcriptional level. Translation level and posttranslational level regulate the expression of genes, which play an important role in various biological processes in domestic animals. The origin, classification and structural characteristics of long chain noncoding RNA were analyzed. Four molecular regulation mechanisms (signal molecule, decoy molecule, lead molecule, scaffold molecule) were used to realize biological function. Five levels of regulating gene expression and the methods of analyzing sequencing data and predicting lncRNA of domestic animals by high-throughput RNA sequencing were reviewed in order to provide a basis for the rational study of lncRNA in domestic animals. Animal lncRNA prediction methods include deep sequencing, transcriptome reconstruction, new transcripts prediction, lncRNA recognition, and lncRNA recognition. The authors used a combination of protein coding potential assessment tool CPAT (coding potential assessment tool) and online sequence coding capability prediction tool CPC (coding potential calculator) in the early stages of the study. The lncRNA, of 68 604 Inner Mongolia Cashmere Goats skin tissue was predicted to enrich the basic data of goat lncRNA transcripts in NONCODE database.
【作者单位】：内蒙古农业大学职业技术学院;内蒙古农业大学动物科学学院内蒙古自治区动物遗传育种与繁殖重点实验室;
【基金】：国家自然科学基金(31560622、31260538、30960246)
【分类号】：Q522;S813

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