疫苗油佐剂在鸡蛋和组织中的残留代谢分析

发布时间：2019-01-07 07:11

【摘要】：试验旨在研究油佐剂疫苗在鸡蛋和组织中残留规律。选用120只1日龄的京粉蛋鸡作为试验对象,每组设12个重复,每重复10只。按照免疫程序推荐剂量注射油佐剂疫苗,全部右侧翅肩肌注。在159、166、180、201、235、271d测定鸡蛋中正烷烃和多环芳烃的残留量,同时在280、330、380、430d测定右侧翅肩肌、左侧胸肌、右侧胸肌、左侧腿肌、右侧腿肌、肝、肾中正烷烃和多环芳烃的残留量。结果表明:矿物油中分子量较小的烷烃,如正十六烷、正十七烷、正十八烷,更易于被机体代谢,残留消除速率较快,也易于扩散到机体其他部位;矿物油进入机体后,在注射部位中残留量最大,通过体循环扩散到机体其他组织、器官中,在肝、肾中的残留量较大,且消除速率较慢;各个采样部位正烷烃和多环芳烃残留量随着时间的变化而降低。鸡蛋中未检测到多环芳烃。矿物油经过机体代谢进入鸡蛋中的量很少,不足以对鸡蛋质量安全造成影响;而它在鸡体内组织、器官中形成较长期的残留,特别是长链烷烃需要更长时间的代谢。
[Abstract]:The aim of the experiment was to study the residue of oil adjuvant vaccine in eggs and tissues. 120 1-day-old Jingfen laying hens were selected as experimental objects with 12 replicates in each group and 10 in each replica. Oil adjuvant vaccine is injected with recommended dose according to immune procedure, all right wing shoulder is injected intramuscularly. The residues of n-alkanes and polycyclic aromatic hydrocarbons (PAHs) in eggs were determined at 159166180201 235271 d, and the residues of n-alkanes and polycyclic aromatic hydrocarbons (PAHs) in the right alar muscle, left pectoral muscle, right pectoral muscle, left leg muscle, right leg muscle, liver and kidney were determined at 280330380430 d. The results showed that alkanes with lower molecular weight, such as n-hexadecane, n-heptadecane and n-octadecane, were more easily metabolized by the body, and the rate of elimination of residues was faster, and it was easy to spread to other parts of the body. After the mineral oil entered into the body, the residual amount in the injection site was the largest, and the residual content in the organs, liver and kidney was larger, and the rate of elimination was slow. The residues of n-alkanes and polycyclic aromatic hydrocarbons (PAHs) decreased with time. No polycyclic aromatic hydrocarbons (PAHs) were detected in eggs. The quantity of mineral oil entering into eggs through organism metabolism is not enough to affect the quality and safety of eggs, but it forms long-term residues in the tissues and organs of chickens, especially the long chain alkanes need to be metabolized for a longer time.
【作者单位】：中国农业科学院家禽研究所;
【基金】：江苏省农业科技自主创新资金项目[CX(16)1008] 扬州市农业前瞻性研究项目(yz2014145) 扬州市家禽质量安全科技服务平台建设项目(yz2015162) 2016国家农产品质量安全风险评估重大专项(GJFP2016007)
【分类号】：S859.84

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