禽腺病毒4型抗体间接ELISA检测方法的建立

发布时间：2019-01-10 12:40

【摘要】：为建立一种快速检测鸡群中禽腺病毒4型(FAd V-4)的血清学方法,本研究利用纯化的FAd V-4中国流行株作为包被抗原,并对各反应条件进行优化,建立了检测FAd V-4血清抗体的间接ELISA方法。FAd V-4抗原与抗新城疫病毒、禽流感病毒、传染性法氏囊病病毒、传染性支气管炎病毒、减蛋综合征病毒阳性血清均无交叉反应,表明该方法具有较好的特异性;批内和批间重复试验的最大变异系数分别为3.735%和4.373%,显示该方法具有较好的稳定性;利用所建立的ELISA方法对40份来自疑似FAd V-4感染病鸡的血清样品进行检测,检测结果与FAd V-4 PCR检测结果符合率为100%。对我国河南、山东、安徽、山西和江苏等不同地区的676份鸡血清样品进行了FAd V-4流行病学调查,结果显示上述省份均存在不同程度的FAd V-4感染,抗体阳性率在69.3%~89.1%。表明该方法为FAd V-4流行病学调查和防控提供了一种快速有效的检测方法。
[Abstract]:In order to establish a rapid serological method for the detection of avian adenovirus type 4 (FAd V-4) in chickens, the purified FAd V-4 Chinese epidemic strain was used as the coating antigen, and the reaction conditions were optimized. An indirect ELISA method for detection of FAd V-4 antibody was established. FAd V-4 antigen and anti-Newcastle disease virus, avian influenza virus, infectious bursal disease virus, infectious bronchitis virus, No cross reaction was found in the positive sera of egg drop syndrome virus, which indicated that the method had good specificity. The maximum coefficients of variation were 3.735% and 4.3733%, respectively, which showed that the method had good stability. 40 serum samples from suspected FAd V-4 infected chickens were detected by using the established ELISA method. The coincidence rate between the detection results and the FAd V-4 PCR detection results was 100%. FAd V-4 epidemiological investigation was carried out in 676 chicken serum samples from Henan, Shandong, Anhui, Shanxi and Jiangsu provinces in China. The results showed that there were different degrees of FAd V-4 infection in these provinces. The positive rate of antibody was 69. 3% and 89. 1%. This method provides a rapid and effective method for epidemiological investigation and prevention and control of FAd V-4.
【作者单位】：河南农业大学牧医工程学院;
【基金】：国家重点研发计划(2016YFD0501102、2016YFD0500802) 河南省高校科技创新团队支持计划(14IRTSTHN015)
【分类号】：S852.65

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