鸭病毒性肝炎活疫苗的耐热冻干工艺策略设计
发布时间：2019-01-14 07:33
【摘要】：【目的】为了提高鸭病毒性肝炎弱毒(DHV)活疫苗的耐热性能。【方法】选用蔗糖、普鲁兰糖、明胶、甘油、磷酸盐等成分设计耐热保护剂组方,根据配方的共晶点、塌陷温度科学设计冻干程序,并比较了不同预冻方式对鸭病毒性肝炎活疫苗的效价影响。【结果】耐热保护剂配方A在37℃10 d、15 d病毒损失分别为0.35、0.61 Lg;对快冻、慢冻以及退火3种不同的冻干方式进行比较,发现对鸭肝炎病毒耐热损失由大到小依次为:退火慢冻快冻;疫苗长期保存期试验结果表明,可在2~8℃可保存2年;采用10倍剂量免疫2日龄雏鸭均未出现死亡,且精神饮食体重增长均正常。【结论】采用A配方与快速冻干冻方式的耐热性能最好,延长保存期并且提高了耐热性能,为动物疫苗耐热新剂型的研发提供技术支撑。
[Abstract]:[objective] in order to improve the heat resistance of duck viral hepatitis attenuated (DHV) vaccine, sucrose, pullulanose, gelatin, glycerol, phosphate and other components were selected to design the formula of heat resistance protectant, according to the eutectic point of the formula, Collapse temperature scientifically designed freeze-drying program, and compared the effects of different pre-freezing methods on the titer of live duck viral hepatitis vaccine. [results] the virus loss of heat-resistant protective agent formula A at 37 鈩，
本文编号：2408461
[Abstract]:[objective] in order to improve the heat resistance of duck viral hepatitis attenuated (DHV) vaccine, sucrose, pullulanose, gelatin, glycerol, phosphate and other components were selected to design the formula of heat resistance protectant, according to the eutectic point of the formula, Collapse temperature scientifically designed freeze-drying program, and compared the effects of different pre-freezing methods on the titer of live duck viral hepatitis vaccine. [results] the virus loss of heat-resistant protective agent formula A at 37 鈩，
本文编号：2408461
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