小花棘豆中毒对和田羊母羊繁殖器官的影响
发布时间：2019-01-16 03:33
【摘要】：试验旨在探讨小花棘豆(Oxytropis glabra DC)中毒对和田羊母羊繁殖器官指数、性激素水平和相关基因mRNA表达量的影响。以小花棘豆中毒和田羊母羊为研究对象,采集血清后屠宰,采集试验羊的丘脑、垂体和卵巢组织,测定其脏器系数,检测血清中促性腺激素释放激素(GnRH)、促卵泡素(FSH)、促黄体素(LH)、雌二醇(E2)和孕酮(P4)含量的变化,并检测各组织中相关繁殖基因的表达。结果显示,小花棘豆中毒和田羊母羊丘脑、垂体和卵巢指数均极显著升高(P0.01),且卵巢表面卵泡数显著下降(P0.05),HE染色表明丘脑神经元细胞固缩、浓染;垂体中细胞核变形、浓染,胞浆减少;卵巢中初级卵母细胞溶解、消失,间质血管扩张。小花棘豆中毒和田羊母羊血清中GnRH、FSH、LH、E2和P4含量均极显著下降(P0.01),丘脑中Kiss-1、GPR54、ERαmRNA,垂体中GnRHR mRNA和卵巢中FSHR、LHR mRNA表达量均极显著下降(P0.01)。结果表明,小花棘豆毒性成分可通过丘脑—垂体—性腺轴影响和田羊母羊的生殖系统。
[Abstract]:The aim of the experiment was to investigate the effects of (Oxytropis glabra DC) poisoning on reproductive organ index, sex hormone level and mRNA expression of related gene in Hetian sheep. Serum samples were collected and slaughtered, thalamus, pituitary and ovary tissues were collected, organ coefficients were measured, and serum gonadotropin releasing hormone (GnRH),) follicle stimulating hormone (FSH),) was detected. Luteinizing hormone (LH), estradiol (E2) and progesterone (P4) were detected and the expression of related reproductive genes was detected. The results showed that the indexes of thalamus, pituitary and ovary were significantly increased (P0.01), and the number of follicles on the surface of ovary decreased significantly (P0.05), HE staining showed that thalamic neurons were pyknotic and stained intensively). The nuclei of the pituitary gland were deformed, stained and cytoplasm decreased, the primary oocytes in the ovary dissolved and disappeared, and the interstitial blood vessels were dilated. The levels of GnRH,FSH,LH,E2 and P4 in serum decreased significantly (P0.01), and the expression of GnRHR mRNA and FSHR,LHR mRNA in Kiss-1,GPR54,ER 伪 mRNA, pituitary and ovary in thalamus decreased significantly (P0.01). The results showed that the toxic components of Acanthopsis cantonensis could affect the reproductive system of Hetian sheep through the axis of thalamus-pituitary-gonad.
【作者单位】： 塔里木大学动物科学学院新疆生产建设兵团塔里木畜牧科技重点实验室;
【基金】：国家自然科学基金(31460678) 国家星火计划项目(2015GA891015)
【分类号】：S858.26
，
本文编号：2409416
[Abstract]:The aim of the experiment was to investigate the effects of (Oxytropis glabra DC) poisoning on reproductive organ index, sex hormone level and mRNA expression of related gene in Hetian sheep. Serum samples were collected and slaughtered, thalamus, pituitary and ovary tissues were collected, organ coefficients were measured, and serum gonadotropin releasing hormone (GnRH),) follicle stimulating hormone (FSH),) was detected. Luteinizing hormone (LH), estradiol (E2) and progesterone (P4) were detected and the expression of related reproductive genes was detected. The results showed that the indexes of thalamus, pituitary and ovary were significantly increased (P0.01), and the number of follicles on the surface of ovary decreased significantly (P0.05), HE staining showed that thalamic neurons were pyknotic and stained intensively). The nuclei of the pituitary gland were deformed, stained and cytoplasm decreased, the primary oocytes in the ovary dissolved and disappeared, and the interstitial blood vessels were dilated. The levels of GnRH,FSH,LH,E2 and P4 in serum decreased significantly (P0.01), and the expression of GnRHR mRNA and FSHR,LHR mRNA in Kiss-1,GPR54,ER 伪 mRNA, pituitary and ovary in thalamus decreased significantly (P0.01). The results showed that the toxic components of Acanthopsis cantonensis could affect the reproductive system of Hetian sheep through the axis of thalamus-pituitary-gonad.
【作者单位】： 塔里木大学动物科学学院新疆生产建设兵团塔里木畜牧科技重点实验室;
【基金】：国家自然科学基金(31460678) 国家星火计划项目(2015GA891015)
【分类号】：S858.26
，
本文编号：2409416
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