回乳期奶牛血清GH、INS、HC、TGF-β1和IGF-1含量变化

发布时间：2019-01-27 17:30

【摘要】：为探究回乳期奶牛血清中生长激素(GH)、胰岛素(INS)、氢化可的松(HC)、转化生长因子β1(TGF-β1)和胰岛素样生长因子1(IGF-1)含量的变化及其相关性。试验选择规模化奶牛场日产奶量为(15.43±2.10)kg,即将干乳的妊娠后期健康中国荷斯坦奶牛20头。干乳开始当天记为第0天,分别采集第0、1、3、5、7、9和11天奶牛尾静脉血,ELISA法检测血清中GH、INS、HC、TGF-β1和IGF-1含量并对结果进行统计学处理,分析其在奶牛回乳过程中的变化规律及其相关性。结果显示,回乳期奶牛血清GH、INS、HC和TGF-β1含量在第0至1天变化不显著(P0.05);血清GH和HC含量第3至11天依次降低且差异均极显著(P0.01),其中血清HC含量第3天极显著高于其余各天(P0.01);血清INS含量第3至11天依次升高且差异均极显著(P0.01);血清TGF-β1含量第3至11天差异性均不显著(P0.05);血清IGF-1含量依次呈阶梯式下降趋势,第0天和第1天、第3天和第5天、第7天和第9天、第9天和第11天之间均差异不显著(P0.05),但第0至1天、第3至5天、第7至11天3阶段之间差异极显著(P0.01)。回乳期奶牛血清GH、INS、TGF-β1和IGF-1含量变化两两间呈极显著相关(P0.01);HC含量变化与GH、INS和IGF-1含量变化均呈极显著相关(P0.01),与TGF-β1含量变化呈显著负相关(P0.05)。
[Abstract]:To investigate the changes and correlation of serum levels of growth hormone (GH), insulin (INS), hydrocortisone (HC), transforming growth factor 尾 1 (TGF- 尾 1) and insulin like growth factor 1 (IGF-1) in the serum of dairy cows during relactation. A total of 20 healthy Chinese Holstein cows with a daily milk yield of (15.43 卤2.10) kg, dry milk were selected. The first day of dry milk was recorded as day 0, and the venous blood samples were collected on day 0 ~ (-1) and day 11 respectively. The contents of GH,INS,HC,TGF- 尾 _ 1 and IGF-1 in serum were detected by ELISA method and the results were analyzed statistically. To analyze the change law and its correlation during the milk returning process of dairy cow. The results showed that there was no significant change in serum GH,INS,HC and TGF- 尾 1 levels between 0 and 1 day after milk resuscitation (P0.05). The levels of serum GH and HC decreased significantly from day 3 to day 11 (P0.01), and the content of serum HC on day 3 was significantly higher than that on the other days (P0.01). The levels of serum INS increased significantly from day 3 to day 11 (P0.01), and the levels of serum TGF- 尾 1 did not differ significantly from day 3 to day 11 (P0.05). The level of serum IGF-1 decreased step by step. There was no significant difference between 0 and 1 day, 3 day and 5 day, 7 day and 9 day, 9 day and 11 day (P0.05), but 0 to 1 day, 3 to 5 days, 3 to 5 days. There were significant differences between the three stages on the 7th and 11th days (P0.01). The changes of serum GH,INS,TGF- 尾 1 and IGF-1 were significantly correlated (P0.01). HC content was significantly correlated with GH,INS and IGF-1 content (P0.01), and negatively correlated with TGF- 尾 1 content (P0.05).
【作者单位】：四川农业大学动物医学院动物疫病与人类健康四川省重点实验室奶牛疾病研究中心;江苏农牧科技职业学院;河北省廊坊市农业局水产站;
【基金】：四川省教育厅重点项目(15ZA0024) 江苏农牧科技职业学院产业发展关键技术创新项目(NSF201604) 四川农业大学双支计划项目
【分类号】：S823

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