治疗奶牛乳房炎的复方中药灌注剂的研究

发布时间：2019-01-27 19:18

【摘要】：奶牛乳腺炎是造成奶牛饲养场经济损失最大的一种常见疾病,因此如何有效的防治奶牛乳房炎已经越来越受到奶牛饲养企业的重视。一直以来,防治奶牛乳房炎的方法大多采用抗生素,随之带来的耐药性和药物残留等问题渐渐暴露出来,中药防治以它无污染、天然和无毒的特性进入人们的视线。本研究选取了几味中药,通过体外抑菌试验确立了最优的提取方法并配制了复方中药乳房灌注剂,对配制好的复方中药乳房灌注剂进行了安全性评价,并在临床上进行了治疗试验。试验结果如下:1查阅文献设计试验,通过体外抑菌试验效果确定最优的提取方法和条件:蒲公英、甘草和当归采用水煮法合并浓缩得到中药提取液,百里香通过挥发油提取器回流提取百里香挥发油,将提取的中草药均匀混合。体外抑菌和MIC的效果说明配制的中药乳房灌注剂对乳房炎的四种常见病原菌有良好的抑制效果。在临床医治奶牛乳房炎上提供了理论和实验基础。2为了评价自制的中药复方乳房灌注剂的安全性,对其进行了粘膜刺激试验与急性毒性试验,粘膜刺激试验结果表示本复方乳房灌注剂对粘膜无刺激性,经口灌服给药时,半数致死量LD50=31063mg/kg,该剂量远小于药物学急性毒性试验规定的标准,所以认为该药物安全无毒,由此可证实本试验复方中药乳房灌注剂剂对小鼠安全无毒,可在临床上安全使用。3试验选择乳房炎奶牛进行分组,分为对照组、中药灌注剂组和抗生素组进行临床治疗试验,观察试验奶牛的临床症状、体温、体细胞数和血清生化指标的变化,得出以下结果:中药乳房灌注剂对乳房炎有一定的治疗效果,替代抗生素使用可以克服抗生素易产生耐药性、药物残留等缺点。
[Abstract]:Cow mastitis is a common disease which causes the biggest economic loss in dairy cattle farm. Therefore, how to effectively prevent and cure cow mastitis has been paid more and more attention by dairy cattle breeding enterprises. The methods of preventing and curing cow mastitis have been mostly used antibiotics, and the problems of drug resistance and drug residue have been exposed gradually. Traditional Chinese medicine has been used to prevent and cure dairy cow mastitis because of its pollution-free, natural and non-toxic characteristics. In this study, several kinds of traditional Chinese medicine were selected, the optimum extraction method was established by in vitro bacteriostasis test, and the breast perfusion agent of compound Chinese medicine was prepared, and the safety of the prepared Chinese herbal medicine breast perfusion agent was evaluated. A therapeutic trial was carried out in clinical practice. The results are as follows: (1) according to the literature design experiment, the optimal extraction method and conditions were determined by in vitro bacteriostasis test: dandelion, Glycyrrhiza and Angelica sinensis were condensed to obtain traditional Chinese medicine extract by water boiling method. The volatile oil of thyme was extracted by reflux of thyme oil extractor, and the extracted Chinese herbal medicine was mixed evenly. The results of in vitro bacteriostasis and MIC showed that the prepared Chinese medicine breast perfusion had a good inhibitory effect on the four common pathogens of mastitis. In order to evaluate the safety of traditional Chinese medicine compound breast perfusion agent, mucosal stimulation test and acute toxicity test were carried out. The results of mucosal stimulation test showed that this compound mammary perfusion agent had no irritation to the mucous membrane. When administered orally, the lethal dose of LD50=31063mg/kg, was far less than the standard prescribed in the pharmacological acute toxicity test, so the drug was considered safe and non-toxic. It can be proved that this compound Chinese medicine breast perfusion agent is safe and non-toxic to mice, and can be safely used in clinic. 3 the experiment selected mastitis cows to be divided into control group, traditional Chinese medicine perfusion group and antibiotic group for clinical treatment. By observing the changes of clinical symptoms, body temperature, somatic cell count and serum biochemical indexes of dairy cows, the following results were obtained: Chinese medicine breast perfusion has a certain therapeutic effect on mastitis. Alternative antibiotic use can overcome the disadvantages of antibiotic resistance and drug residue.
【学位授予单位】：宁夏大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.23

【参考文献】