长期饲喂高脂饲料对大鼠血脂、肝及肠道菌群的影响

发布时间：2019-02-15 08:00

【摘要】：旨在探讨长期饲喂高脂饲料对大鼠血脂、肝及肠道菌群的影响。本研究选择成年健康SD大鼠随机分为两组:空白对照组和高脂饲料组(n=10),分别采用维持饲料和高脂饲料饲喂13周,在第8及第13周,采集血清进行血脂分析,并于第13周采集肝组织及盲肠内容物,检测肝病变及肠道菌群变化,并预测与其相关的代谢通路。结果表明,高脂饲料可显著升高TG、TCH及LDL水平(P0.01),并导致严重的肝脂肪变性;相比于第8周结果,延长饲喂时间可显著增高TCH和HDL(P0.01)。高脂饲料可显著降低肠道菌群Shannon及Chao指数(P0.001),降低菌群多样性,并显著改变菌群组成,引起厚壁菌门及放线菌门的增加和拟杆菌门的减少(P0.01);PICRUSt预测对照组差异代谢通路,主要涉及细菌的生存与繁殖等,而高脂饲料组主要与能量、氨基酸代谢相关,这些通路的改变可能与血脂异常及肠道菌群多样性的降低紧密联系。本研究证实了长期饲喂高脂饲料对大鼠血脂、肝及肠道菌群的危害,为血脂异常成因的阐明提供了依据,也对宠物健康饮食管理有一定的指导作用。
[Abstract]:The aim of this study was to investigate the effects of high fat diet on blood lipids, liver and intestinal flora in rats. In this study, adult healthy SD rats were randomly divided into two groups: blank control group and high fat diet group (nr10). The rats were fed with maintenance diet and high fat diet for 13 weeks, and serum lipid levels were analyzed at the 8th and 13th weeks. The contents of liver tissue and cecum were collected at the 13th week to detect the changes of hepatic lesions and intestinal flora and to predict the metabolic pathways associated with them. The results showed that high fat diet could significantly increase the levels of TG,TCH and LDL (P0.01) and lead to severe hepatic steatosis, and the prolongation of feeding time could significantly increase TCH and HDL (P0.01) compared with the results of the 8th week. High fat diet significantly decreased the Shannon and Chao index (P0.001) of intestinal flora, decreased the diversity of flora, and significantly changed the composition of flora, resulting in the increase of phylum and actinomycetes and the decrease of Bacteroides (P0.01). PICRUSt predicted that the differential metabolic pathways in the control group were mainly involved in the survival and reproduction of bacteria, while those in the high-fat diet group were mainly related to the metabolism of energy and amino acids. The changes of these pathways may be closely related to the abnormal blood lipid and the decrease of the diversity of intestinal flora. This study confirmed the harm of long-term feeding high fat diet on blood lipids, liver and intestinal microflora in rats, provided the basis for elucidating the causes of dyslipidemia, and also had a certain guiding effect on the management of healthy diet for pets.
【作者单位】：中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室;
【基金】：国家自然科学基金(31402254;31572573) 甘肃省青年科技基金计划(1506RJYA148)
【分类号】：S816

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