PCV2病毒生物矿化条件的优化
发布时间：2019-02-16 10:32
【摘要】：以矿化效率为评价标准,对猪圆环病毒2型(PCV2)生物矿化的反应温度、反应时间及Ca/P比、抗原初始p H、添加剂用量等条件进行优化,并通过小鼠实验检测矿化病毒的免疫原性。结果显示,生物矿化最优处理条件为初始p H 6.5,Ca/P 1.67,4℃矿化12 h,Mg2+/Ca2+为1.0。在该反应条件下,病毒粒子能够形成球形结构的钙磷PCV2粒子,粒径为200~500 nm。不同批次PCV2抗原矿化率皆在90%以上,且该方法可去除大量杂蛋白。小鼠免疫矿化PCV2病毒颗粒后14~28 d,抗体水平与商品苗对照组、未矿化PCV2病毒组基本相同,28 d后对照组抗体水平下降明显,而PCV2矿化颗粒组的抗体基本维持在原水平,表明PCV2矿化颗粒具有缓释抗原的作用。
[Abstract]:The reaction temperature, reaction time and Ca/P ratio of porcine circovirus type 2 (PCV2) biomineralization, the initial antigen pH, the dosage of additives and so on were optimized. The immunogenicity of mineralized virus was detected by mouse experiment. The results showed that the optimal treatment conditions for biomineralization were as follows: initial pH 6.5 Ca / P 1.67 鈩，
本文编号：2424353
[Abstract]:The reaction temperature, reaction time and Ca/P ratio of porcine circovirus type 2 (PCV2) biomineralization, the initial antigen pH, the dosage of additives and so on were optimized. The immunogenicity of mineralized virus was detected by mouse experiment. The results showed that the optimal treatment conditions for biomineralization were as follows: initial pH 6.5 Ca / P 1.67 鈩，
本文编号：2424353
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