猪繁殖与呼吸综合征病毒感染猪肺泡巨噬细胞天然免疫信号通路相关分子变化分析
发布时间：2019-02-19 17:26
【摘要】：利用蛋白质组学的方法对猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)感染猪肺泡巨噬细胞(pulmonary macrophage,PAM)后的蛋白质动态变化进行探讨,为阐明病毒感染后引起的复杂免疫学反应及为该病的防控提供理论依据。通过Label-free LC-MS/MS方法定量鉴定PRRSV感染PAMs后差异表达细胞蛋白质。结果显示,共鉴定出430个显著差异表达蛋白质,其中包括171个上调表达蛋白质和259个下调表达蛋白质,这些蛋白质与细胞抗病毒天然免疫及抗原递呈等相关。值得关注的是,许多上调表达蛋白质都富集在与天然免疫相关的NF-κB、MAPK及RIG-Ⅰ等信号通路上,包括STAT1/2、OAS1/2、Mx1/2、ISG15/20、IFIT1/2/3/5等;下调蛋白质主要富集在MHCⅠ和MHCⅡ分子相关信号通路上。荧光定量PCR和Western blot结果证实,PRRSV感染导致了STAT1/2、OAS1/2、Mx1、CD14、ISG和IFIT等的转录或表达,这为进一步阐述PRRSV的感染机制奠定基础。
[Abstract]:The dynamic changes of protein in porcine alveolar macrophages (pulmonary macrophage,PAM) infected with porcine reproductive and respiratory syndrome virus (porcine reproductive and respiratory syndrome virus,PRRSV) were studied by proteomics. In order to clarify the complex immune response caused by virus infection and to provide a theoretical basis for the prevention and control of the disease. Differential expression of cellular proteins after PRRSV infection with PAMs was quantitatively identified by Label-free LC-MS/MS. The results showed that 430 differentially expressed proteins were identified, including 171 up-regulated proteins and 259 down-regulated proteins, which were related to cellular antiviral innate immunity and antigen presentation. It is worth noting that many up-regulated proteins are concentrated in NF- 魏 B map-MAPK and RIG- I signaling pathways related to innate immunity, including STAT1/2,OAS1/2,Mx1/2,ISG15/20,IFIT1/2/3/5. The down-regulated proteins are mainly concentrated in MHC 鈪，
本文编号：2426703
[Abstract]:The dynamic changes of protein in porcine alveolar macrophages (pulmonary macrophage,PAM) infected with porcine reproductive and respiratory syndrome virus (porcine reproductive and respiratory syndrome virus,PRRSV) were studied by proteomics. In order to clarify the complex immune response caused by virus infection and to provide a theoretical basis for the prevention and control of the disease. Differential expression of cellular proteins after PRRSV infection with PAMs was quantitatively identified by Label-free LC-MS/MS. The results showed that 430 differentially expressed proteins were identified, including 171 up-regulated proteins and 259 down-regulated proteins, which were related to cellular antiviral innate immunity and antigen presentation. It is worth noting that many up-regulated proteins are concentrated in NF- 魏 B map-MAPK and RIG- I signaling pathways related to innate immunity, including STAT1/2,OAS1/2,Mx1/2,ISG15/20,IFIT1/2/3/5. The down-regulated proteins are mainly concentrated in MHC 鈪，
本文编号：2426703
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