艾叶对热应激蛋鸡抗氧化功能、产蛋性能和蛋品质的影响

发布时间：2019-02-21 17:01

【摘要】：为了评价艾叶缓解蛋鸡热应激效应及适宜添加水平,本研究测定了艾叶提取物中抗氧化成分含量及体外抗氧化性能,研究了艾叶对热应激条件下蛋鸡抗氧化功能、产蛋性能和蛋品质的影响。试验选用720只38周龄海兰褐蛋鸡,随机分为6组,每组6个重复,每重复20只,即常温对照组、高温对照组、常温艾叶组、高温低艾叶组、高温中艾叶组、高温高艾叶组。常温和高温对照组饲喂基础饲粮,常温艾叶组在基础饲粮中添加3.0%艾叶粉,高温艾叶组分别在基础饲粮中添加1.0%、2.0%、3.0%艾叶粉。预试期为7d,正试期21d,记录生产性能指标并测定鸡蛋品质,每7d测定1次血清抗氧化及应激指标。结果显示,艾叶具有较高的抗氧化成分和清除自由基能力,总黄酮含量为87.78mg/g,总多酚含量为126.57mg/g,维生素C含量为3.12 mg/g,清除羟基自由基、超氧阴离子自由基及DPPH自由基的半数有效剂量分别为356.86μg/mL、76.84μg/mL、293.67μg/mL。在常温条件下,蛋鸡日粮中添加艾叶对蛋鸡生产性能、产蛋性能无显著影响(P0.05);在高温应激条件下,蛋鸡采食量和产蛋率显著降低,料蛋比升高(P0.05),在蛋鸡饲料中添加不同水平的艾叶14d时,与高温对照组相比,蛋鸡采食量无显著变化(P0.05),血清超氧化物歧化酶和谷胱甘肽过氧化物酶活力显著提高,丙二醛及皮质醇含量显著降低(P0.05);产蛋率提高及料蛋比降低(P0.05),且能显著改善蛋黄颜色、降低胆固醇含量(P0.05)。试验结果表明,艾叶具有较好的体内外抗氧化能力,在热应激条件下蛋鸡日粮中添加2%的艾叶可以一定程度增强蛋鸡抗热应激能力,提高蛋鸡产蛋性能并改善鸡蛋品质。
[Abstract]:In order to evaluate the effect of Artemisia argyi leaf on relieving heat stress and its suitable addition level, the antioxidative components in the extract of Artemisia argyi leaf and its antioxidant activity in vitro were measured, and the antioxidative function of Artemisia argyi leaf on heat stress was studied. Effects of egg laying performance and egg quality. 720 Hailan brown laying hens aged 38 weeks were randomly divided into 6 groups with 6 replicates in each group, namely, normal temperature control group, high temperature control group, normal temperature Artemisia argyi group, high temperature low Aiya leaf group, high temperature and high temperature Artemisia argyi group, and high temperature Artemisia argyi group. The normal temperature and high temperature control groups were fed with basic diet. The normal temperature and high temperature control groups were fed with 3.0% Ai leaf powder in the basic diet and 1.0% 2.0% and 3.0% Ai leaf powder respectively in the base diet of the high temperature Artemisia argyi group. The pre-test period was 7 days and the positive test period was 21 days. The production performance index and egg quality were recorded and serum antioxidant and stress indexes were measured once every 7 days. The results showed that the leaves had high antioxidant and free radical scavenging ability. The total flavonoids content was 87.78 mg / g, the total polyphenol content was 126.57 mg / g, and the vitamin C content was 3.12 mg/g, to scavenge hydroxyl free radical, the total flavonoids content was 87.78 mg / g, the total polyphenol content was 126.57 mg / g, the vitamin C content was 3.12 mg/g,. The effective doses of superoxide anion radical and DPPH radical were 356.86 渭 g / mL ~ 76.84 渭 g / m ~ (-1) and 293.67 渭 g / m ~ (-1), respectively. Under the condition of normal temperature, there was no significant effect on laying performance and laying performance of laying hens by adding Artemisia lanceolata to the diet (P0.05). Under the condition of high temperature stress, the feed intake and laying rate of laying hens decreased significantly, and the feed to egg ratio increased (P0.05). After 14 days of adding different levels of Ai leaf into the feed of laying hens, there was no significant change in the feed intake of laying hens compared with the control group (P0.05). The activities of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH) increased significantly, and the contents of malondialdehyde (MDA) and cortisol decreased significantly (P0.05). The egg production rate and feed / egg ratio decreased (P0.05), and significantly improved egg yolk color and decreased cholesterol content (P0.05). The results showed that Artemisia argyi had better antioxidant ability in vivo and in vitro. Adding 2% of Artemisia argyi to the diet of laying hens under heat stress could enhance the ability of resisting heat stress, improve laying performance and improve egg quality to a certain extent.
【作者单位】：河南牧业经济学院;江西省农业科学院畜牧兽医研究所;新希望六和股份有限公司;
【基金】：江西省科技支撑重点项目(20151BBF60063)
【分类号】：S831.5

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