猪伪狂犬病毒gB、gC和gD蛋白多抗制备及交叉中和抗体效价测定
发布时间：2019-03-05 11:59
【摘要】：为研究猪伪狂犬病毒(PRV)主要免疫原性基因的抗原变异情况,本研究构建了PRV变异株JS-2012和弱毒疫苗株Bartha-K61的g B、g C和g D基因重组真核表达质粒。通过基因枪途径免疫新西兰大白兔,对制备的兔源多克隆抗体进行交叉中和抗体效价的测定。结果显示,JS-2012和Bartha-K61株的g C蛋白和g D蛋白多抗对PRV JS-2012变异株、SC经典强毒株和Bartha-K61弱毒疫苗株的交叉中和效价均无显著差异;而两个病毒株的g B蛋白多抗对JS-2012和SC株的中和效价较低,分别为1∶29.0~56.7和1∶56.2~137.0,对Bartha-K61株以及其他毒力基因缺失弱毒疫苗株的中和效价较高,分别为1∶75.0~490.0和1∶198.6~986.9,差异显著,推测该结果可能与PRV毒力基因缺失有关。本研究为进一步鉴定PRV流行株的抗原变异奠定基础。
[Abstract]:In order to study the antigen variation of major immunogenic genes of porcine pseudorabies virus (PRV), the recombinant eukaryotic expression plasmids of GB, GC and GD genes of PRV variant JS-2012 and attenuated vaccine strain Bartha-K61 were constructed. New Zealand white rabbits were immunized by gene gun. The cross neutralizing antibody titer of rabbit-derived polyclonal antibody was determined. The results showed that there was no significant difference in cross neutralization titer between JS-2012 strain and Bartha-K61 strain against PRV JS-2012 variant strain, SC classical virulent strain and Bartha-K61 attenuated vaccine strain. The neutralization titers of g B protein polyclonal antibody against JS-2012 and SC strains were lower than those of Bartha-K61 strain and SC strain, respectively, and the neutralization titers to Bartha-K61 strain and other virulence gene deletion weak virus vaccine strain were higher than those of Bartha-K61 strain and other virulence gene deletion weak virus vaccine strain, respectively, and the neutralization titers of GB protein polyclonal antibody against Bartha-K61 strain and other virulent genes were higher than those of other virulent genes. The results were 1? 75.0? 490.0 and 1? 198.6? 986.9, respectively. The difference was significant, which suggested that the result might be related to the deletion of virulence gene in PRV. This study lays a foundation for further identification of antigen variation of PRV epidemic strains.
【作者单位】： 中国农业科学院上海兽医研究所;
【基金】：上海市自然科学基金项目(14ZR1448900) 上海市科技兴农重点攻关项目[沪农科攻字(2015)第1-7号] 中央级公益性科研院所基本科研业务费专项资金项目(2014JB02)
【分类号】：S852.4
本文编号：2434884
[Abstract]:In order to study the antigen variation of major immunogenic genes of porcine pseudorabies virus (PRV), the recombinant eukaryotic expression plasmids of GB, GC and GD genes of PRV variant JS-2012 and attenuated vaccine strain Bartha-K61 were constructed. New Zealand white rabbits were immunized by gene gun. The cross neutralizing antibody titer of rabbit-derived polyclonal antibody was determined. The results showed that there was no significant difference in cross neutralization titer between JS-2012 strain and Bartha-K61 strain against PRV JS-2012 variant strain, SC classical virulent strain and Bartha-K61 attenuated vaccine strain. The neutralization titers of g B protein polyclonal antibody against JS-2012 and SC strains were lower than those of Bartha-K61 strain and SC strain, respectively, and the neutralization titers to Bartha-K61 strain and other virulence gene deletion weak virus vaccine strain were higher than those of Bartha-K61 strain and other virulence gene deletion weak virus vaccine strain, respectively, and the neutralization titers of GB protein polyclonal antibody against Bartha-K61 strain and other virulent genes were higher than those of other virulent genes. The results were 1? 75.0? 490.0 and 1? 198.6? 986.9, respectively. The difference was significant, which suggested that the result might be related to the deletion of virulence gene in PRV. This study lays a foundation for further identification of antigen variation of PRV epidemic strains.
【作者单位】： 中国农业科学院上海兽医研究所;
【基金】：上海市自然科学基金项目(14ZR1448900) 上海市科技兴农重点攻关项目[沪农科攻字(2015)第1-7号] 中央级公益性科研院所基本科研业务费专项资金项目(2014JB02)
【分类号】：S852.4
【相似文献】
相关会议论文 前4条
1 毛群颖;郭增兵;李清;梁争论;李凤祥;王军志;;2010年我国人免疫球蛋白中EV71中和抗体效价的比较研究[A];2010年中国药学大会暨第十届中国药师周论文集[C];2010年
2 侯继锋;孙思才;王敏力;王威;杨靖清;;献浆员中EV71中和抗体效价分布研究与分析[A];2010年中国药学大会暨第十届中国药师周论文集[C];2010年
3 秦婷婷;刘兰军;杨春;李小姣;刘瑞熙;武志强;张航;刘波;;四川地区供浆员中EV71中和抗体效价的分布研究[A];2011中国生物制品年会暨第十一次全国生物制品学术研讨会论文集[C];2011年
4 杨春;刘兰军;高昌茜;刘瑞熙;秦婷婷;容新宗;张航;;四川地区供浆员中CMV中和抗体效价分布状况研究[A];2011中国生物制品年会暨第十一次全国生物制品学术研讨会论文集[C];2011年
相关硕士学位论文 前1条
1 刘新建;SARS-CoV实验灭活疫苗免疫效果评价及体外抗SARS-CoV药物研究[D];暨南大学;2004年
，本文编号：2434884
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2434884.html
