枸杞鲜花总多酚抗氧化性研究

发布时间：2019-03-12 18:18

【摘要】：试验以枸杞鲜花为试材,采用微波法提取其中多酚并进行体外抗氧化作用的研究以溶剂乙醇浓度、提取时间、微波功率作为枸杞鲜花多酚提取试验的主要影响因素,依据回归分析法确定出多酚提取得率以作为响应值的单因素响应面和等高线,分析因素的显著性和交互作用进而优化提取工艺,并将提取得到的枸杞鲜花多酚进行体外化学抗氧化性试验。结果表明,枸杞鲜花多酚的最佳提取工艺:乙醇浓度78.6%,提取时间149 s,微波功率63.2 W,多酚得率为0.057 4%;枸杞鲜花多酚还原力FRAP值为497.9/μl;对DPPH的IC50值为1.4701mg/l;对ABTS~+的IC_(50)值为0.178 6 mg/l;对NO_2~-的IC_(50)值为0.413 3 mg/l。
[Abstract]:In the experiment, Lycium barbarum flower was used as the test material, the polyphenol was extracted by microwave method and the antioxidant effect in vitro was studied. The concentration of solvent ethanol, the extraction time and the microwave power were the main influencing factors of the extraction test of Lycium barbarum flower polyphenol. According to regression analysis, the extraction rate of polyphenols was determined as single factor response surface and contour as response value, and the significance and interaction of factors were analyzed to optimize the extraction process. And the extracted polyphenols from Lycium barbarum flower were tested for chemical antioxidation in vitro. The results showed that the optimum extraction technology of polyphenol from Lycium barbarum flower was as follows: ethanol concentration 78.6%, extraction time 149s, microwave power 63.2W, polyphenol yield 0.057, reducing power of Lycium barbarum flower polyphenol 497.9 / 渭 l, polyphenol reducing power of Lycium barbarum flower was 497.9 / 渭 l. IC 50 for DPPH is 1.4701 mg / ml, IC_ (50) for ABTS~ is 0.178 6 mg/l; for NO_2~-, IC_ (50) for NO_2~- is 0.413 3 mg/l.
【作者单位】：北方民族大学生物科学与工程学院;
【基金】：宁夏自然科学基金项目[NZ14102]
【分类号】：S816

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