小尾寒羊与新吉细毛羊羊毛及毛囊性状比较研究

发布时间：2019-03-20 13:14

【摘要】：为进一步验证小尾寒羊与新吉细毛羊毛用性状与毛囊性状差异。本研究选择9月龄小尾寒羊与新吉细毛羊母羊各8只,在相同饲养环境下测定毛用性状及毛囊性状并分析两者间的差异,最后用定量PCR方法检测不同月份2个品种绵羊毛囊周期分子的表达变化趋势。结果表明:小尾寒羊在主要毛用性状,包括产毛量(GFW)、毛细度(FD)、拉伸长度(SL)、卷曲度及油脂率等大部分指标均极显著低于新吉细毛羊(P0.01);组织切片法测定小尾寒羊与新吉细毛羊毛囊制表显示,小尾寒羊毛囊密度、S/P值等极显著低于新吉细毛羊(P0.01),但在皮肤厚度、初级毛囊、次级毛囊毛乳头直径等显著高于新吉细毛羊;毛囊周期标记分子定量PCR显示,不同季节小尾寒羊与新吉细毛羊LEF1和TGF-β1基因总体变化趋势相同,但小尾寒羊变化更为剧烈,说明气候等外界因素对小尾寒羊毛囊周期影响大于新吉细毛羊。本研究证明小尾寒羊与新吉细毛羊的毛用性状、毛囊性状存在极显著差异,TGF-β1为进一步探讨小尾寒羊与新吉细毛羊毛用性状表型差异分子遗传基础研究奠定基础。
[Abstract]:In order to further verify the small tail Han sheep and new fine wool characteristics and hair follicles of the difference. In this study, 8 ewes of 9-month-old small tail Han sheep and Xinji fine wool sheep were selected. The wool traits and hair follicle traits were measured under the same feeding environment and the differences between them were analyzed. Finally, quantitative PCR was used to detect the change trend of hair follicle cycle molecule expression in two sheep breeds in different months. The results showed that most of the main wool traits of small tail Han sheep, including wool yield (GFW), capillary degree (FD), stretch length (SL), curl degree and fat percentage, were significantly lower than those of Xinji fine wool sheep (P0.01). Tissue sectioning method showed that the density and S / P ratio of small tail Han sheep hair follicles were significantly lower than that of Xinji fine wool sheep (P0.01), but the skin thickness, primary hair follicles, and so on, were significantly lower than those of small tail Han sheep and Xinji fine wool sheep (P0.01). The diameter of secondary follicular papilla was significantly higher than that of Xinji fine wool sheep. The results of quantitative PCR showed that the changes of LEF1 and TGF- 尾 1 genes in small tail Han sheep and Xinji fine wool sheep were the same in different seasons, but the changes in small tail Han sheep were more severe than those in Xinji fine wool sheep. The results showed that climate and other external factors affected the cycle of hair follicles of small tail Han sheep more than that of Xinji fine wool sheep. The results showed that there were significant differences in wool traits and follicles between small tail Han sheep and new fine wool sheep. TGF- 尾 1 laid the foundation for further study on molecular genetic basis of phenotypic differences between small tail Han sheep and new fine wool sheep.
【作者单位】：吉林省农业科学院畜牧分院;延边大学农学院;
【基金】：国家863计划(2013AA102506)
【分类号】：S826

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