不同来源木聚糖酶及其组合对木聚糖水解产物组成、细菌增殖与大肠杆菌黏附性的影响

发布时间：2019-03-25 06:34

【摘要】：本试验旨在研究不同来源木聚糖酶及其组合对木聚糖的水解效果,并研究不同木聚糖水解产物对细菌增殖及大肠杆菌对肠道上皮细胞黏附性的影响。试验用木聚糖酶A和木聚糖酶B分别来源于毕赤酵母和米曲霉。采用木聚糖酶A、木聚糖酶B、组合酶1(木聚糖酶A∶木聚糖酶B=3∶7)、组合酶2(木聚糖酶A∶木聚糖酶B=1∶1)、组合酶3(木聚糖酶A∶木聚糖酶B=7∶3)分别水解木聚糖,然后测定木聚糖水解产物对大肠杆菌、枯草芽孢杆菌和乳酸杆菌增殖和大肠杆菌对肠道上皮细胞黏附性的影响。结果表明:1)2种木聚糖酶有一定的组合效应,木聚糖酶A、木聚糖酶B、组合酶1、组合酶2、组合酶3组木二糖和木三糖的总含量分别为95.70%、86.79%、93.11%、94.55%和87.55%,其中木聚糖酶A组木二糖含量最高,组合酶2组木三糖含量最高。2)培养20 h时,5种木聚糖水解产物对大肠杆菌的增殖(以菌液吸光度值表示)没有产生显著影响(P0.05);培养20和30 h时,5种木聚糖水解产物显著促进枯草芽孢杆菌的增殖(P0.05);培养13和17 h时,5种木聚糖水解产物显著促进乳酸杆菌的增殖(P0.05)。3)5种木聚糖水解产物均可以显著降低大肠杆菌对肠道上皮细胞的黏附率(P0.05)。由此可见,通过不同来源木聚糖酶及其组合水解木聚糖,可以产生以木二糖和木三糖为主要组分的水解产物,从而起到促进枯草芽孢杆菌和乳酸杆菌增殖、减少大肠杆菌对肠道上皮细胞黏附的作用。
[Abstract]:The aim of this study was to study the hydrolysis of xylan by xylanase from different sources and the effects of xylanase hydrolysate on bacterial proliferation and adhesion of Escherichia coli to intestinal epithelial cells. Xylanase A and B were derived from Pichia pastoris and Aspergillus oryzae respectively. Xylanase A, xylanase B, combination enzyme 1 (xylanase A: xylanase B), and xylanase 2 (xylanase A: xylanase 1 / 1), xylanase A, xylanase B, xylanase A and xylanase B, were used. Xylanase 3 (xylanase A: xylanase B) was used to hydrolyze xylan, and then the effects of xylan hydrolysate on the proliferation of Escherichia coli, Bacillus subtilis and Lactobacillus subtilis and the adhesion of Escherichia coli to intestinal epithelial cells were determined. The results showed that: 1) the total contents of xylanase A, xylanase B, combination enzyme 1, combination enzyme 2, combination enzyme 3 were 95.70% and 86.79%, respectively, and xylanase A, xylanase B, xylose and xylose were 95.70% and 86.79%, respectively. 93.11%, 94.55% and 87.55%, among which the xylanase A group had the highest xylose content and the combination enzyme 2 group had the highest xylose content. 2) when cultured for 20 h, the xylanase A group had the highest xylose content. 5 xylan hydrolysate had no significant effect on the proliferation of Escherichia coli (expressed by the absorbance value of bacterial liquid) (P0.05). 5 xylan hydrolysate significantly promoted the proliferation of Bacillus subtilis at 20 h and 30 h (P0.05). When cultured for 13 and 17 h, 5 xylan hydrolysate significantly promoted the proliferation of Lactobacillus (P0.05). 3) all of the five xylan hydrolysate could significantly reduce the adhesion rate of E. coli to intestinal epithelial cells (P0.05). It can be seen that xylose and xylose can be hydrolyzed by xylanase from different sources and combined with xylanase, which can promote the proliferation of Bacillus subtilis and Lactobacillus subtilis. Reduce the effect of E. coli on intestinal epithelial cell adhesion.
【作者单位】：华南农业大学动物科学学院;广东温氏食品集团有限公司;
【基金】：广东省科技计划项目(2016A020210104) 广州市科技计划项目(201510010258)
【分类号】：S816

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