蒲公英水提物对猪链球菌生物被膜体外干预作用

发布时间：2019-03-25 10:54

【摘要】：旨在观察蒲公英水提物对猪链球菌的抑菌作用及其对生物被膜形成的干预作用。通过水提法提取蒲公英的活性成分,利用高效液相色谱法检测蒲公英水提物中绿原酸的含量。通过微量肉汤稀释法分别测定蒲公英水提物和绿原酸对猪链球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);利用结晶紫染色(CV)和扫描电镜(SEM)检测蒲公英水提物和绿原酸对猪链球菌生物被膜形成的影响。结果表明,蒲公英水提物中绿原酸含量为5.833mg·mL-1;蒲公英水提物对猪链球菌的MIC和MBC分别为62.5和250mg·mL-1;绿原酸对猪链球菌MIC和MBC分别为2.5和10mg·mL-1。扫描电镜观察可见阴性对照组细菌镶嵌于生物被膜中,细菌周围有厚厚的黏液层,而蒲公英水提物和绿原酸均能使细菌生物被膜中的细菌数量和生物被膜的形态发生明显变化,细菌数量明显减少,未见有黏液成分和胞外基质,二者对猪链球菌生物被膜的形成均有抑制作用,均呈剂量依赖性。结果提示,蒲公英水提物对体外猪链球菌生物被膜形成具有抑制作用,并呈剂量依赖性;蒲公英水提物对体外猪链球菌生物被膜形成抑制作用的主要活性成分为绿原酸。
[Abstract]:The aim of this study was to observe the bacteriostatic effect of dandelion extract on Streptococcus suis and its intervention on biofilm formation. The active components of dandelion were extracted by water extraction and the content of chlorogenic acid in water extract of Taraxacum dandelion was determined by high performance liquid chromatography (HPLC). Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC);) of dandelion extract and chlorogenic acid against Streptococcus suis were determined by broth dilution method. The effects of dandelion extract and chlorogenic acid on biofilm formation of Streptococcus suis were detected by crystal violet staining (CV) and scanning electron microscopy (SEM). The results showed that the content of chlorogenic acid in water extract of dandelion was 5.833mg 路mL-1, the MIC and MBC of water extract of dandelion to Streptococcus suis were 62.5 and 250mg 路mL-1, respectively. The MIC and MBC values of chlorogenic acid against Streptococcus suis were 2.5 and 10mg 路mL-1, respectively. Scanning electron microscopy (SEM) showed that the bacteria in negative control group were embedded in biofilm and there was thick mucus layer around the bacteria. The water extract of dandelion and chlorogenic acid could obviously change the number of bacteria in biofilm and the morphology of biofilm. The number of bacteria decreased obviously and no mucus and extracellular matrix were found. Both of them inhibited the formation of Streptococcus suis biofilm in a dose-dependent manner. The results suggested that the water extract of dandelion could inhibit the formation of Streptococcus suis biofilm in vitro in a dose-dependent manner, and the main active component of dandelion water extract in inhibiting biofilm formation of Streptococcus suis in vitro was chlorogenic acid.
【作者单位】：东北农业大学动物医学学院;东北农业大学动物科学技术学院;
【基金】：黑龙江省博士后基金(LBH-Z11243) 北京市重点实验室开放课题(TCVW-201301)
【分类号】：S853.7

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