刈割期及添加剂对苜蓿青贮品质及CNCPS蛋白组分的影响

发布时间：2019-03-26 14:01

【摘要】：本试验旨在研究生物及化学添加剂对不同时期刈割的苜蓿青贮品质的影响。试验采用现蕾期和初花期刈割的中苜一号苜蓿(70%水分含量)为原料,分别设置添加:1)空白组;2)乳酸菌+纤维素酶(10~5 cfu/g+50mg/kg);3)乳酸菌+纤维素酶(10~5 cfu/g+100mg/kg);4)甲酸+丙酸(6mL/kg)4个处理组,使用0.5L塑料桶调制罐装青贮,并于发酵30d后开罐取样分析。结果表明,与现蕾期相比,初花期苜蓿青贮饲料的乳酸(LA)产量较高,丁酸(BA)和氨态氮(NH_3-N)生成量较低(P0.01),pH值也较低(P0.05),同时非蛋白氮(PA)和结合蛋白质(PC)含量也显著低于现蕾期(P0.01)。3个添加剂处理组均显著地提高了青贮发酵的品质,降低pH值(P0.01)和氨态氮生成量(P0.01)。其中,乳酸菌+纤维素酶显著地提高了乳酸含量及乳酸/乙酸(LA/AA)(P0.01),甲酸+丙酸则显著抑制了丁酸的产生(P0.01)。同时,3组添加剂均显著地提高了青贮饲料中可溶性碳水化合物(WSC)和粗蛋白(CP)的含量,并降低了中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的含量(P0.01)。就蛋白组分而言,乳酸菌+纤维素酶(10~5 cfu/g+100mg/kg)和甲酸+丙酸处理组显著地降低了青贮饲料中非蛋白氮和结合蛋白质的含量(P0.01),提高了真蛋白(PB)的含量(P0.01)。综合而言,甲酸+丙酸处理组苜蓿青贮料的品质最佳,乳酸菌+纤维素酶(10~5 cfu/g+100mg/kg)次之。
[Abstract]:The purpose of this experiment was to study the effects of biological and chemical additives on the quality of alfalfa silage at different cutting stages. In the experiment, alfalfa Zhongmu No. 1 (70% water content) was used as raw material, 1) blank group, 2) lactobacillus cellulase (10? 5 cfu/g 50mg/kg), 2) lactobacillus cellulase (10? 5 mol / L), 2) lactobacillus cellulase (10? 5%); 3) Lactobacillus cellulase (10 渭 5 cfu/g 100mg/kg) and 4) formic acid propionic acid (6mL/kg) groups, the silage was prepared in a 0.5L plastic barrel, and the samples were collected and analyzed after 30 days of fermentation. The results showed that the yield of lactic acid (LA), the production of butyric acid (BA) and ammonia nitrogen (NH_3-N) in alfalfa silage at early flowering stage were higher than those in bud stage (P0.01), and the pH value was also lower (P0.05). At the same time, the contents of non-protein nitrogen (PA) and binding protein (PC) were significantly lower than those of bud stage (P0.01). Decrease pH value (P0.01) and ammonia nitrogen production (P0.01). Among them, lactic acid bacteria cellulase significantly increased lactic acid content and lactic acid / acetic acid (LA/AA) (P0.01), formic acid propionic acid significantly inhibited the production of butyric acid (P0.01). At the same time, the contents of soluble carbohydrate (WSC) and crude protein (CP) in silage were significantly increased and the contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were decreased (P0.01). In terms of protein components, lactic acid bacteria cellulase (10? 5 cfu/g 100mg/kg) and formic acid propionic acid significantly reduced the contents of non-protein nitrogen and binding proteins in silage (P0.01). The content of true protein (PB) was increased (P0.01). Generally speaking, the quality of alfalfa silage treated with formic acid propionic acid was the best, followed by lactobacillus cellulase (10? 5 cfu/g 100mg/kg).
【作者单位】：中国农业大学动物科技学院;沈阳农业大学园艺学院;鄂尔多斯市农牧业科学研究院;
【基金】：国家牧草产业技术体系(CARS-35) 公益性行业(农业)科研专项(201303061)(201403048) 天津市农业科技成果转化与推广项目(201404040) 内蒙古自治区科技计划项目(苜蓿混合青贮调制技术研究与示范)资助
【分类号】：S816.53

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