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应用CNCPS方法和体外产气法研究豆腐渣饲料的营养价值

发布时间:2019-03-28 20:48
【摘要】:为揭示豆腐渣对反刍动物的饲用价值,本试验采用康奈尔净碳水化合物—蛋白质体系(CNCPS)和体外产气法对豆腐渣营养价值进行评定。测定豆腐渣的CNCPS营养成分与体外发酵参数,应用CNCPS公式计算碳水化合物(CHO)和粗蛋白质(CP)中各组分含量。结果显示,豆腐渣的CP含量为14%~25%,粗脂肪(EE)含量为1.9%~2.9%,代谢能(ME)为10~12 MJ/kg DM;易在瘤胃中降解的蛋白质(PA+PB1)和在瘤胃中缓慢降解的粗蛋白质(PB2+PB3)的含量相当,不可降解真蛋白质(PC)含量较少;潜在可利用碳水化合物(CA+CB1+CB2)较高;体外发酵产气量均达到70mL,氨态氮(NH3-N)浓度在8~23mg/dL,总挥发性脂肪酸(TVFA)在46~70mmol/L。而不同来源的豆腐渣营养成分和发酵情况存在显著差异,且CNCPS碳水化合物、蛋白质组分的优劣不同。凌源豆腐渣的最大产气量(B)最高,极显著高于其他3种苹果渣(P0.01),而各豆腐渣的产气速度(C)无显著差异(P0.05)。辽源豆腐渣氨态氮(NH3-N)含量最高,极显著高于其他饲料(P0.01)。不同地方豆腐渣瘤胃液挥发性脂肪酸比例差异极显著(P0.01)。不同来源的豆腐渣的总可消化养分(TDN)及其他能值差异较大,以新民豆腐渣的能值最低,极显著低于其他苹果渣(P0.01)。综上可知,作为反刍动物非粮饲料,豆腐渣为反刍动物提供的可利用能值高,利于瘤胃微生物蛋白质合成。
[Abstract]:In order to reveal the feeding value of bean curd residue for ruminants, Cornell's net carbohydrate-protein system (CNCPS) and in vitro gas production method were used to evaluate the nutritional value of soybean curd residue. The nutrient composition of CNCPS and fermentation parameters in vitro were determined. The contents of carbohydrate (CHO) and crude protein (CP) were calculated by CNCPS formula. The results showed that the content of CP in dregs of bean curd was 14% and 25%, the content of (EE) in crude fat was 1.9% and 2.9%, and the metabolizable energy (ME) was 10? 12 MJ/kg DM;. The content of protein (PA PB1) easily degraded in rumen was similar to that of crude protein (PB2 PB3) which degraded slowly in rumen, and the content of (PC) in non-degradable real protein was lower, and the potential available carbohydrate (CA CB1 CB2) was higher. The in vitro gas production was 70 mL, the concentration of ammonia nitrogen (NH3-N) was 8-23 mg / L, and the total volatile fatty acid (TVFA) was 46-70 mmol 路L ~ (- 1) 路h ~ (- 1). There were significant differences in nutrient composition and fermentation status of soybean curd residue from different sources, and the advantages and disadvantages of CNCPS carbohydrate and protein components were different. The maximum gas production rate (B) of Lingyuan bean curd dregs was the highest, which was significantly higher than that of the other three kinds of apple dregs (P0.01), but there was no significant difference in the gas production rate (C) of each bean curd dregs (P0.05). The content of ammonia nitrogen (NH3-N) in Liaoyuan bean curd residue was the highest, which was significantly higher than that of other diets (P0.01). There was significant difference in the proportion of volatile fatty acids in rumen juice of bean curd residue between different places (P0.01). The total digestible nutrients (TDN) and other energy values of soybean curd residue from different sources were significantly different. The energy value of Xinmin dregs was the lowest, which was significantly lower than that of other apple dregs (P0.01). In conclusion, as a non-food feed for ruminants, bean curd residue provides high available energy for ruminants, which is beneficial to rumen microbial protein synthesis.
【作者单位】: 中国农业大学动物科技学院动物营养学国家重点实验室;
【基金】:国家自然科学基金面上项目(31372335) 国家肉牛牦牛产业技术体系项目(CARS-38) 公益性行业(农业)科研专项经费项目(201503134)
【分类号】:S816


本文编号:2449217

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