饲喂高精料日粮对奶牛泌乳及相关调节因子的影响
发布时间：2019-03-29 18:45
【摘要】：为了解高精料日粮对泌乳奶牛乳产量的影响,选择12只健康经产奶牛,分别饲喂精粗比为40∶60(对照组)和60∶40(高精料组)的日粮,试验期12周,每周监测乳产量。试验期间每周采集血液样品用于测定外周血生长激素(GH)和胰岛素样生长因子1(IGF-1)含量;试验结束,采集瘤胃液置-70℃保存待测,肝脏和乳腺组织于液氮速冻后置-70℃保存待测。结果表明,高精料日粮的饲喂显著降低了瘤胃pH(P0.05);奶牛外周血GH含量和IGF-1含量从第7周开始显著降低(P0.05);肝脏组织中生长激素受体(GHR)、蛋白质酪氨酸激酶2(JAK2)、信号传导与转录激活子5A(STAT5A)及信号传导与转录激活子5B(STAT5B)mRNA表达水平显著下调(P0.05);乳腺组织中,胰岛素样生长因子1受体(IGF-1R)mRNA表达水平显著下调(P0.05)。结果提示,长期饲喂高精料日粮会降低血液中GH和IGF-1含量,抑制GH-IGF-1轴的基因表达及关联的JAK2-STAT5信号途径的相关基因表达,从而减少了乳产量。
[Abstract]:In order to understand the effect of high concentrate diet on milk yield of lactating cows, 12 healthy dairy cows were fed with the diets of 40:60 (control group) and 60:40 (high concentrate group), respectively. The milk yield was monitored weekly during the experiment period of 12 weeks. The peripheral blood growth hormone (GH) and insulin-like growth factor-1 (IGF-1) were measured by sampling blood samples every week during the experiment. At the end of the experiment, the rumen fluid was collected and stored at-70 鈩，
本文编号：2449787
[Abstract]:In order to understand the effect of high concentrate diet on milk yield of lactating cows, 12 healthy dairy cows were fed with the diets of 40:60 (control group) and 60:40 (high concentrate group), respectively. The milk yield was monitored weekly during the experiment period of 12 weeks. The peripheral blood growth hormone (GH) and insulin-like growth factor-1 (IGF-1) were measured by sampling blood samples every week during the experiment. At the end of the experiment, the rumen fluid was collected and stored at-70 鈩，
本文编号：2449787
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