乳酸菌复合制剂对柔嫩艾美耳球虫感染雏鸡免疫水平的影响及免疫保护效果评价

发布时间：2019-03-29 20:09

【摘要】：为探究乳酸菌等益生菌在雏鸡抗柔嫩艾美耳球虫(Eimeria tenella)感染中的作用,本研究以150只1日龄肉雏鸡为试验动物,以2×10~8CFU/200 L鼠李糖乳杆菌、乳酸片球菌和植物乳杆菌复合制剂饲喂雏鸡,并设红、白对照组。于攻虫前后检测细胞因子IFN-γ和IL-2、IgG和SIgA水平,雏鸡血液中淋巴细胞增殖情况和外周血CD4~+和CD8~+T淋巴细胞数量,并对攻虫期间增重情况、卵囊排出数量和盲肠病变情况进行检测。结果显示,该制剂显著提高了IFN-γ、IL-2、IgG、SIgA水平和外周血中T淋巴细胞数量,从而增强了机体细胞和体液免疫水平(P0.05)。此外,该制剂可以显著减轻球虫感染对雏鸡增重的影响,降低卵囊排出量和盲肠病理损伤(P0.05),ACI值达到中等抗球虫效果,有望成为鸡球虫病新的防治手段。
[Abstract]:In order to investigate the effect of probiotics such as lactic acid bacteria on anti-(Eimeria tenella) infection of Eimeria tenella, 150 one-day-old chicks were used as experimental animals and 2 脳 10~8CFU/200 / L rhamnose lactobacillus were used as experimental animals. Lactococcus lactobacillus and Lactobacillus plantarum compound preparation was fed to chicks, and red and white control group. The levels of cytokines IFN- 纬 and IL-2,IgG and SIgA, the proliferation of lymphocytes in blood and the number of CD4~ and CD8~ T lymphocytes in peripheral blood of chicks were measured before and after infestation, and the weight gain during infestation was also observed. The quantity of oocyst efflux and cecum lesion were detected. The results showed that the preparation significantly increased the levels of IFN- 纬, IL-2,IgG,SIgA and the number of T lymphocytes in peripheral blood, thus enhancing the cellular and humoral immune levels (P0.05). In addition, the preparation can significantly reduce the effect of coccidiosis infection on chicken weight gain, reduce the amount of oocyst efflux and cecal pathological injury (P0.05), and the ACI value can reach a moderate anti-coccidiosis effect, which is expected to become a new means of prevention and treatment of coccidiosis in chickens.
【作者单位】：吉林农业大学动物科学技术学院吉林省动物微生态制剂工程研究中心;
【基金】：国家高技术研究发展计划(863)项目(2013AA102806) 国家自然科学基金项目(31272552,31272541,31672528) 吉林省科技发展计划项目(20160519011JH) 吉林省产业创新专项资金项目(2016C063)
【分类号】：S858.31

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