外源酶对湖羊瘤胃发酵的影响
发布时间：2019-04-01 13:57
【摘要】：本实验主要研究外源酶对湖羊瘤胃液微生物体外培养影响,外源酶是由纤维素酶和木聚糖酶按照4：1比例配制。研究外源酶对瘤胃液体外培养影响,旨在为外源酶作为湖羊饲料添加剂开发提供理论依据。采用湖羊瘤胃液体外培养方法研究外源酶10mg/lg、20mg/kg、 30mg/kg和40mg/kg四个水平,分别培养6h、12h、18h、24h、36h和48h后对体外培养液中pH值、氨态氮(NH3-N)浓度、微生物蛋白(MCP)浓度和挥发性脂肪酸(VFA)浓度影响。结果表明,添加不同水平外源酶均能改善瘤胃微生物体外培养特性,其中以添加量为20mg/kg处理组效果最佳,VFA比对照组提高37%(P0.01),NH3-N浓度比对照组提高17%(P0.01),MCP比对照组提高8%(P0.01)。可见添加外源酶可改善瘤胃微生物发酵特性。
[Abstract]:The aim of this study was to study the effects of exogenous enzymes on the in vitro culture of microorganisms in rumen fluid of Hu sheep. The exogenous enzymes were prepared by cellulase and xylanase in the proportion of 4:1. The effect of exogenous enzyme on rumen liquid culture in vitro was studied in order to provide theoretical basis for the development of exogenous enzyme as feed additive for Hu sheep. Four levels of exogenous enzyme (10 mg / kg, 20 mg / kg, 30mg/kg and 40mg/kg) were studied in vitro culture of Hu sheep rumen fluid. The concentrations of pH value, ammonia nitrogen (NH3-N) and ammonia nitrogen (NH3-N) in culture medium were cultured for 6 h, 12 h, 18 h, 24 h, 36 h and 48 h, respectively, at the levels of 10 mg / kg, 20 mg / kg, 6 h, 12 h, 18 h, 24 h, respectively. The effect of microbial protein (MCP) concentration and volatile fatty acid (VFA) concentration. The results showed that adding different levels of exogenous enzymes could improve the characteristics of rumen microbial culture in vitro, in which the addition of 20mg/kg was the best, and the VFA was 37% higher than that of the control group (P0.01). The concentration of NH3-N was increased by 17% (P0.01) compared with the control group (P0.01), 8% higher than that of the control group (P0.01). It can be seen that the addition of exogenous enzymes can improve the fermentation characteristics of rumen microorganisms.
【学位授予单位】：福建农林大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S826.5
本文编号：2451611
[Abstract]:The aim of this study was to study the effects of exogenous enzymes on the in vitro culture of microorganisms in rumen fluid of Hu sheep. The exogenous enzymes were prepared by cellulase and xylanase in the proportion of 4:1. The effect of exogenous enzyme on rumen liquid culture in vitro was studied in order to provide theoretical basis for the development of exogenous enzyme as feed additive for Hu sheep. Four levels of exogenous enzyme (10 mg / kg, 20 mg / kg, 30mg/kg and 40mg/kg) were studied in vitro culture of Hu sheep rumen fluid. The concentrations of pH value, ammonia nitrogen (NH3-N) and ammonia nitrogen (NH3-N) in culture medium were cultured for 6 h, 12 h, 18 h, 24 h, 36 h and 48 h, respectively, at the levels of 10 mg / kg, 20 mg / kg, 6 h, 12 h, 18 h, 24 h, respectively. The effect of microbial protein (MCP) concentration and volatile fatty acid (VFA) concentration. The results showed that adding different levels of exogenous enzymes could improve the characteristics of rumen microbial culture in vitro, in which the addition of 20mg/kg was the best, and the VFA was 37% higher than that of the control group (P0.01). The concentration of NH3-N was increased by 17% (P0.01) compared with the control group (P0.01), 8% higher than that of the control group (P0.01). It can be seen that the addition of exogenous enzymes can improve the fermentation characteristics of rumen microorganisms.
【学位授予单位】：福建农林大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S826.5
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