广东新型鸭呼肠孤病毒σB蛋白基因克隆及序列分析

发布时间：2019-04-11 12:21

【摘要】：为研究广东新型呼肠孤病毒(NDRV)的基因变异及遗传演化情况,本试验从广东不同鸭场病死鸭肝脏、脾脏等组织脏器中分离到8株流行毒株,用RT-PCR方法进行σB蛋白基因扩增、克隆与序列分析,并与其他毒株σB蛋白的氨基酸特性、蛋白抗原和蛋白基因进行系统进化比对分析。结果表明,广东省鸭群中感染的NDRV与国内其他地区报道的DRV核苷酸序列同源性很高,达96.6%~99.5%,而与禽呼肠孤病毒(ARV)和番鸭呼肠孤病毒(MDRV)的同源性则较低,分别为64.6%~66.5%和66.2%~67.1%;8株NDRV之间的核苷酸序列同源性高达97.7%~99.7%。与其他毒株比较结果显示,本试验分离的NDRV毒株磷酸化位点均比参考毒株ARV和MDRV少,且大多数区域的抗原指数都较高,抗原性与MDRV较为接近,遗传进化分析结果表明,NDRV和国内其他DRV处于独立的进化分支,ARV和MDRV则处于不同分支。结果表明,广东省流行的NDRV毒株σB蛋白基因序列高度保守,且广东地区分离的NDRV与国内其他地区分离的DRV没有明显的地域差异。
[Abstract]:In order to study the genetic variation and genetic evolution of a new type of reovirus (NDRV) in Guangdong Province, 8 strains of epidemic strains were isolated from the liver, spleen and other tissues of dead ducks in different duck farms in Guangdong Province. The 蟽 B protein gene was amplified by RT-PCR, cloned and sequenced, and compared with the amino acid characteristics, protein antigen and protein gene of other strains. The results showed that the nucleotide sequences of NDRV infected in ducks in Guangdong province were highly homologous to those reported in other regions of China, reaching 96.6% and 99.5% respectively, while the homology with avian reovirus (ARV) and muscovy duck reovirus (MDRV) was lower than that of poultry reovirus (ARV) and muscovy duck reovirus (MDRV). 64.6% / 66.5% and 66.2% / 67.1%, respectively. The nucleotide sequence homology of 8 NDRV strains was as high as 97.7% and 99.7% respectively. Compared with other strains, the phosphorylated sites of NDRV strains isolated in this study were lower than those of reference strains ARV and MDRV, and the antigen index of most regions was higher, and the antigenicity was close to that of MDRV. NDRV and other domestic DRV are independent evolutionary branches, while ARV and MDRV are in different branches. The results showed that the 蟽 B protein gene sequence of NDRV strain was highly conserved in Guangdong province, and there was no obvious regional difference between the NDRV isolated from Guangdong region and the DRV isolated from other regions in China.
【作者单位】：佛山科学技术学院生命科学与工程学院;
【基金】：基金项目:广东省家禽重大传染病控制技术研究(2012A020100001)
【分类号】：S852.65

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