不同剂量褪黑素对多浪羊受胎及产羔效果的影响

发布时间：2019-04-13 11:32

【摘要】：【目的】研究褪黑素对多浪羊受胎及产羔效果的影响,提高多浪羊繁殖率。【方法】研究注射褪黑素提高受胎率和产羔率的方法,选择年龄、胎次、体况相似的57只多浪羊母羊,试验羊随机分为三组同时进行同期发情处理,并在撤栓36 h后皮下注射褪黑素注射剂。试验1组1 mL(5 mg)、试验2组2 mL(10 mg)、对照组0 mL。并在埋栓前(中午12:00)、撤栓时(中午12:00)、撤栓后36 h(晚上24:00)、配种时(撤栓后48 h,中午12:00)、配种后20 d五个时间段采集血清,采用放射免疫法对其褪黑素含量进行测定与分析;配种后第45d采用B超监测法检测三组的受胎率;在产羔后比较这三组的产羔率。【结果】试验1组受胎率显著高于试验2组和对照组(P0.05),试验2组与对照组之间差异不显著(P0.05);对产羔率而言,三组之间相互比较后无统计学差异(P0.05)。【结论】对多浪羊进行同期发情处理时,撤栓后36 h皮下注射1、2 mL褪黑素会提高受胎率,其中1 mL的受胎率高于2 mL,但是对产羔率无显著影响。
[Abstract]:[objective] to study the effect of melatonin on the fecundity and lambing of Dolang sheep, and to improve the reproduction rate of Dorang sheep. [methods] to study the method of increasing the conception rate and lambing rate by injection of melatonin, and to select the age and the number of births. 57 Dolang ewes in similar condition were randomly divided into three groups at the same time for estrus treatment. Melatonin injection was injected subcutaneously 36 hours after withdrawal of suppository. Test 1, 1 mL (5 mg), 2 mL, 10 mg), control group, 0 mL.) And before implantation (12:00), when the thrombus was removed (12:00), 36 hours (24:00) after thrombus removal, 48 hours after the thrombus removal, 12:00 at noon, and five time periods 20 days after the implantation, the serum was collected at the time of removal of the thrombus (24:00 in the evening), at 48 hours after the removal of the thrombus, and at 12:00 at noon. The content of melatonin was determined and analyzed by radioimmunoassay. The conception rate of the three groups was detected by B-ultrasound monitoring method on the 45th day after mating. [results] the conception rate of experiment 1 group was significantly higher than that of test 2 group and control group (P0.05), but there was no significant difference between test 2 group and control group (P0.05). For lambing rate, there was no significant difference among the three groups (P0.05). [conclusion] Subcutaneous injection of melatonin at 36 h after withdrawal of thrombus could increase the conception rate of Dulang sheep (P0.05). [conclusion] when Dulang sheep were treated with the same estrus, 2 mL melatonin could increase the conception rate at 36 h after thrombus withdrawal. The conception rate of 1 mL was higher than 2 mL, but had no significant effect on lambing rate.
【作者单位】：新疆农业大学动物科学学院;中国农业大学动物科技学院;
【基金】：新疆维吾尔自治区科技支疆项目“”(2016E02037) 国家星火计划项目“”(2015GA890002) 中国农业大学-新疆农业大学联合基金项目“”(2015TC050)~~
【分类号】：S826

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