微贮玉米秸秆对道寒杂交断奶羔羊瘤胃发酵及微生物区系的影响

发布时间：2019-04-19 13:12

【摘要】：本试验旨在研究微贮玉米秸秆对道寒杂交断奶羔羊瘤胃发酵及微生物区系的影响,为微贮玉米秸秆促进营养物质消化吸收研究提供理论依据。试验选用平均体重为(24.62±3.59)kg的道寒杂交断奶羔羊80只,采用单因子完全随机试验设计分为5组(每组16只),分别饲喂以微贮玉米秸秆替代0(对照)、25%、50%、75%、100%青贮玉米秸秆的全混合日粮(TMR)。预试期15 d,正试期60 d。结果表明:在饲喂4 h后,瘤胃液p H除25%添加组较对照组稍有下降(P0.05)外,50%、75%、100%添加组分别较对照组提高了1.39%(P0.05)、4.16%(P0.05)、7.45%(P0.01);随着微贮玉米秸秆添加量的增加,瘤胃液总挥发性脂肪酸的含量呈先上升后降低趋势,25%、50%、75%添加组分别较对照组升高了0.93%(P0.05)、4.18%(P0.05)、5.19%(P0.05),100%添加组与对照组相近(P0.05);各添加组瘤胃液乙酸/丙酸均低于对照组,25%、50%、75%、100%添加组分别较对照组下降了12.44%(P0.05)、4.93%(P0.05)、12.21%(P0.05)、9.86%(P0.05)。变性梯度凝胶电泳(DGGE)图谱分析得出,微贮玉米秸秆替代适量青贮玉米秸秆促进了瘤胃液纤维降解菌的增殖,抑制了蛋白质降解菌的增殖。综上可知,以微贮玉米秸秆替代75%青贮玉米秸秆配制TMR更有利于提高道寒杂交断奶羔羊的瘤胃发酵水平。
[Abstract]:The purpose of this experiment was to study the effect of microstorage corn straw on rumen fermentation and microbial flora of crossbred weanling lambs, and to provide a theoretical basis for the study of micro-storage corn straw to promote nutrient digestion and absorption. 80 weaned crossbred lambs with average body weight of (24.62 卤3.59) kg were randomly divided into 5 groups (16 lambs in each group), fed with corn straw in microstorage instead of 0 (control), 25%, respectively, in a single-factor complete randomized trial, and 16 lambs in each group were randomly divided into 5 groups (n = 16). Full mixed diets of 50%, 75%, 100% silage corn stalks, (TMR). The pre-trial period is 15 days and the trial period is 60 d. The results showed that after 4 hours of feeding, the rumen juice pH decreased slightly (P0.05), 50%, 75% and 100% increased by 1.39% (P0.05) in addition to 25%, 75% and 100% of rumen juice, respectively, compared with the control group (P0.05). 4.16% (P0.05), 7.45% (P0.01); The content of total volatile fatty acids in rumen juice increased at first and then decreased with the increase of the amount of corn straw in microstorage, 25%, 50% and 75% respectively increased by 0.93% compared with the control group (P0.05), and the content of total volatile fatty acids in rumen juice increased by 0.93% (P0.05). 4.18% (P0.05), 5.19% (P0.05), 100% additive group was similar to the control group (P0.05); The ratio of acetic acid / propionic acid in rumen fluid of each addition group was lower than that of control group, 25%, 50%, 75%, and 100%, respectively, decreased by 12.44% (P0.05), 4.93% (P0.05), 12.21% (P0.05), respectively, compared with the control group. 9.86% (P0.05). The results of denaturing gradient gel electrophoresis (DGGE) showed that the replacement of corn straw with microstorage promoted the proliferation of fiber-degrading bacteria in rumen juice and inhibited the proliferation of protein-degrading bacteria. In conclusion, replacing 75% silage corn straw with micro-storage corn straw to prepare TMR is more beneficial to improve rumen fermentation level of crossbred weanling lambs.
【作者单位】：河北农业大学动物科技学院;河北农业大学生命科学学院;
【基金】：国家肉羊现代产业技术体系建设项目(CARS-39) 国家绒毛用羊产业技术体系建项目(CARS-40-21)
【分类号】：S826.5

【相似文献】