叶酸对炔雌醚致雄性大鼠生殖损伤的保护作用研究

发布时间：2019-04-21 07:49

【摘要】：旨在探讨叶酸对炔雌醚致雄性大鼠生殖损伤的保护作用。将20只8周龄成年雄性SD大鼠适应饲养1周后,随机分为4组。对照组:橄榄油+生理盐水;炔雌醚组:1.0mg·kg-1炔雌醚;炔雌醚+叶酸组:1.0mg·kg-1炔雌醚+1.1mg·kg-1叶酸;叶酸组:1.1mg·kg-1叶酸;灌胃处理,每天一次,连续2周。处理结束后,取生殖器官称重,检测精子质量,测定睾丸抗氧化功能,制作睾丸组织切片,观察睾丸组织结构,通过免疫组织化学染色检测Caspase-3与eNOS表达。结果显示,炔雌醚单独处理组造成大鼠生殖器官萎缩,重量下降,精子发生异常,精子质量下降,抗氧化酶活性下降,睾丸发生氧化应激;与之相反,凋亡效应酶Caspase-3与eNOS表达增加。炔雌醚+叶酸处理组通过减少eNOS表达降低氧化应激,抑制Caspase-3表达,降低精子畸形率、生精小管损伤,最终改善雄性大鼠生殖功能。与对照组相比,单独叶酸处理组睾丸抗氧化酶活性、组织结构和精子质量均有一定程度提高,无显著差异。本研究首次证实叶酸有效地抑制了炔雌醚对雄性大鼠生殖功能的破坏作用。
[Abstract]:The aim of this study was to investigate the protective effect of folic acid on reproductive injury induced by estryne in male rats. Twenty 8-week-old adult male SD rats were randomly divided into 4 groups after one week of acclimatization. Control group: olive oil saline; estradiol group: 1.0mg kg-1; folic acid group: 1.1mg kg-1 folate; folic acid group: 1.1mg kg-1 folate; Intragastric administration, once a day for 2 weeks. After the treatment, the reproductive organs were taken to weigh, the quality of sperm was measured, the antioxidation function of testis was determined, the sections of testis were made, the structure of testis was observed, and the expression of Caspase-3 and eNOS was detected by immunohistochemical staining. The results showed that the reproductive organ atrophy, weight decrease, sperm abnormal, sperm quality decreased, antioxidant enzyme activity decreased and testicular oxidative stress occurred in rats treated with estradiol alone. On the contrary, the expression of Caspase-3 and eNOS increased. Through reducing the expression of eNOS, inhibiting the expression of Caspase-3, reducing the rate of sperm deformity and damaging the seminiferous tubule, the reproductive function of male rats was improved by reducing the expression of Estradiol and folic acid. Compared with the control group, the antioxidant enzyme activity, tissue structure and sperm quality of the testis in the folic acid treated group were improved to a certain extent, and there was no significant difference between the two groups. This study for the first time confirmed that folic acid effectively inhibited the damage of estradiol to reproductive function in male rats.
【作者单位】：河南科技大学动物科技学院;北京昌平区中西医结合医院;中国农业大学动物医学院;
【基金】：国家自然科学基金项目(U1404325;31302106) 河南省教育厅高等学校重点科研项目基础研究计划(16A230004) 河南科技大学青年科学基金项目(2015QN032);河南科技大学高级别科研项目培育基金(序号4) 河南省自然科学基金项目(162300410081)
【分类号】：S859.7

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