北川白山羊FABP3基因cDNA的克隆及表达研究

发布时间：2019-04-26 08:43

【摘要】：脂肪酸结合蛋白(Fatty aicd Binding Protein 3,FABP3)是细胞内脂类伴侣。本研究采用RT-PCR和RACE技术,分离并克隆山羊FABP3基因的cDNA序列,结合实时定量分析山羊FABP3在10个不同组织和5个不同泌乳时期的相对表达。结果表明:FABP3基因的m RNA序列全长为714 bp,包括5'非翻译区64 bp,CDS区402 bp和3'非翻译区248 bp,共计翻译为133个氨基酸,山羊FABP3与Gen Bank中牛(Bos taurus)、小鼠(Mus musculus)和人(Homo sapiens)的核苷酸同源性较高,分别为96%、89%、87%;蛋白质结构分析显示,FABP3蛋白分子量为14.76 ku,等电点为6.11,不具有信号肽序列,没有跨膜结构;组织表达分析表明,FABP3基因在山羊的心脏组织中表达量最高,小肠次之,表达量最少的为肌肉组织;山羊5个不同泌乳时期乳腺组织FABP3的表达量分析表明,泌乳盛期的表达量最高,约为干奶期的130倍。
[Abstract]:Fatty acid binding protein (Fatty aicd Binding Protein 3 (FABP 3) is an intracellular lipid chaperone. In this study, RT-PCR and RACE techniques were used to isolate and clone the cDNA sequence of goat FABP3 gene. The relative expression of goat FABP3 in 10 different tissues and 5 different lactation stages was analyzed by real-time quantitative analysis. The results showed that the full length of m RNA sequence of FABP3 gene was 714 bp, including 5 'untranslated region 64 bp,CDS region 402 bp and 3' untranslated region 248 bp,. Goat FABP3 and bovine (Bos taurus), in Gen Bank were translated into 133amino acids. The nucleotide homology between mouse (Mus musculus) and human (Homo sapiens) was 96%, 89% and 87%, respectively. The protein structure analysis showed that the molecular weight of FABP3 protein was 14.76 ku, isoelectric point 6.11, there was no signal peptide sequence and no transmembrane structure. Tissue expression analysis showed that the expression of FABP3 gene in goat heart tissue was the highest, followed by small intestine, and the lowest expression was in muscle tissue. The expression level of FABP3 in the breast tissues of goat at different lactation stages was the highest, which was about 130 times as high as that at dry milk stage.
【作者单位】：运城农业职业技术学院;四川省畜牧科学研究院;
【基金】：国家科技支撑计划(2011BAD28B01)
【分类号】：S827.2

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