捕食性真菌Duddingtonia flagrans基因组DNA的提取及基因组survey分析
发布时间：2019-04-28 20:58
【摘要】：采用透析膜培养法结合DNA柱式提取法进行基因组DNA提取,同时利用第2代高通量测序技术,分析了捕食性真菌Duddingtonia flagrans基因组概况。结果显示:透析膜培养结合DNA柱式提取法所提取的基因组DNA质量及浓度均能达到测序标准;捕食性真菌Duddingtonia flagrans基因组约为37.6 Mb,GC含量为44.95%,基因组杂合度较低。结果表明:透析膜培养法结合柱式法提取其基因组DNA的方法简便可行,为丝状真菌DNA提取,提供了新的思路;基因组survey分析为捕食性真菌Duddingtonia flagrans全基因组精细图谱的绘制奠定了基础。
[Abstract]:The genomic DNA was extracted by dialysis membrane culture combined with DNA column extraction, and the genomic profile of predatory fungi Duddingtonia flagrans was analyzed by the second generation high throughput sequencing technique. The results showed that the quality and concentration of genomic DNA extracted by dialysis membrane culture combined with DNA column extraction could meet the sequencing standard, and the genomic DNA content of predatory fungi Duddingtonia flagrans was about 37.6 Mb,GC and 44.95%, and the heterozygosity of genome was low. The results showed that the method for extracting genomic DNA from filamentous fungi by dialysis membrane culture combined with column method was simple and feasible, which provided a new way to extract DNA from filamentous fungi. Genomic survey analysis laid a foundation for mapping the whole genome fine map of predatory fungi Duddingtonia flagrans.
【作者单位】： 内蒙古农业大学兽医学院/农业部动物疾病临床诊疗技术重点实验室;内蒙古农牧业科学院;
【基金】：国家自然科学基金资助项目(31460656)
【分类号】：S852.66
本文编号：2467965
[Abstract]:The genomic DNA was extracted by dialysis membrane culture combined with DNA column extraction, and the genomic profile of predatory fungi Duddingtonia flagrans was analyzed by the second generation high throughput sequencing technique. The results showed that the quality and concentration of genomic DNA extracted by dialysis membrane culture combined with DNA column extraction could meet the sequencing standard, and the genomic DNA content of predatory fungi Duddingtonia flagrans was about 37.6 Mb,GC and 44.95%, and the heterozygosity of genome was low. The results showed that the method for extracting genomic DNA from filamentous fungi by dialysis membrane culture combined with column method was simple and feasible, which provided a new way to extract DNA from filamentous fungi. Genomic survey analysis laid a foundation for mapping the whole genome fine map of predatory fungi Duddingtonia flagrans.
【作者单位】： 内蒙古农业大学兽医学院/农业部动物疾病临床诊疗技术重点实验室;内蒙古农牧业科学院;
【基金】：国家自然科学基金资助项目(31460656)
【分类号】：S852.66
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