延边黄牛黄体中凋亡相关基因的表达研究
发布时间：2019-05-10 00:39
【摘要】：为了研究细胞因子在延边黄牛黄体中表达变化本研究采用HE染色法、RT-PCR分析,免疫印迹分析等实验方法,分别观察了cFLIP、Bcl-2及Bax这三种基因在延边黄牛不同时期黄体中的表达,得到以下结果：1、HE染色结果,可观察到随着黄体退化、引起黄体细胞结构性凋亡。2、对处于发情期的牛黄体内cFLIP mRNA的表达量,用RT-PCR技术进行研究,结果表明,cFLIP mRNA在初期、中期、后期和白体中表达较高。3、抗凋亡基因cFLIP蛋白,Bcl-2蛋白的表达量在黄体初期和中期较高,处于后期和黄体期的基因表达量较低。
[Abstract]:In order to study the expression of cytokines in Yanbian yellow yolk, cFLIP, was observed by HE staining, RT-PCR analysis and immunoblotting analysis. The expression of Bcl-2 and Bax genes in the corpus luteum of Yanbian cattle at different stages was studied. The following results were obtained: 1. The results of HE staining showed that the structural apoptosis of luteal cells was caused by the degeneration of corpus luteum. The expression of cFLIP mRNA in estrus was studied by RT-PCR technique. The results showed that the expression of, cFLIP mRNA was higher in the early, middle, late and white bodies. 3, anti-apoptotic gene cFLIP protein. The expression of Bcl-2 protein was higher in the early and middle corpus, but lower in the late stage and the corpus phase.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823
本文编号：2473219
[Abstract]:In order to study the expression of cytokines in Yanbian yellow yolk, cFLIP, was observed by HE staining, RT-PCR analysis and immunoblotting analysis. The expression of Bcl-2 and Bax genes in the corpus luteum of Yanbian cattle at different stages was studied. The following results were obtained: 1. The results of HE staining showed that the structural apoptosis of luteal cells was caused by the degeneration of corpus luteum. The expression of cFLIP mRNA in estrus was studied by RT-PCR technique. The results showed that the expression of, cFLIP mRNA was higher in the early, middle, late and white bodies. 3, anti-apoptotic gene cFLIP protein. The expression of Bcl-2 protein was higher in the early and middle corpus, but lower in the late stage and the corpus phase.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823
【参考文献】
相关期刊论文 前2条
1 王旭有;;延边黄牛产业优势与劣势分析[J];企业技术开发;2010年21期
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，本文编号：2473219
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