复合益生菌对小鼠和家兔生长性能以及肠道调理作用的影响
发布时间：2019-05-20 07:21
【摘要】：在生产中使用的复合益生菌大多是外源性的活菌制剂,随后的研究发现低聚糖能够选择性提高动物肠道内固有益生菌群的增殖,与活菌制剂产生的益生效果相似。功能性低聚糖在动物胃肠道内不能直接消化利用,但却能被肠道的益生菌利用,同时具有抑制有害菌的生长,因而对动物胃肠道微生物区系、免疫功能等有重要影响。植物多糖饱含丰富的营养成分和活性物质,具有抗菌、抗病毒、抗氧化、提高动物机体免疫机能等功能,对机体健康大有裨益。近年来,中国畜牧业发展取得了举世瞩目的成就,但随之而来的药物残留等问题也严重影响着畜产品的消费以及出口,研发混和益生菌添加剂成为近几年畜禽业研究的方向之一。鉴于前人相关的研究,本课题通过低聚木糖和泰山松花粉多糖对植物乳杆菌体外增殖研究发现在植物乳杆菌菌液中添加20mg/mL的低聚木糖和50mg/m L的泰山松花粉多糖后菌落数显著上升;添加20mg/m L低聚木糖和50mg/mL泰山松花粉多糖后在pH=5以上的培养基中植物乳杆菌生长良好,活菌数较其他添加量组高;植物乳杆菌在人工模拟胃液中培养1h活菌数基本不受影响,培养3h后活菌数大约降低10%;在人工模拟肠液中培养3h活菌数不变。结果表明,植物乳杆菌具备益生菌特性,在菌液中添加20mg/mL低聚木糖和50mg/mL泰山松花粉多糖后益生效果更佳明显,可进一步研究其在动物营养和日粮中的应用效果。选取体重25g左右SPF昆明小鼠75只,随机分为5组。Ⅰ组(低聚木糖+泰山松花粉多糖+植物乳杆菌)、Ⅱ组(低聚木糖+植物乳杆菌)、Ⅲ组(泰山松花粉多糖+植物乳杆菌)IV组(沙门氏菌+金黄色葡萄球菌)和Ⅴ组(去离子水)。每组小鼠每天分别灌胃1m L。试验期为期28d,在第7、14、21和28d每组随机选取3只小鼠进行空腹称重,剖杀小鼠后采集盲肠并称重,采集小肠组织分析其肠绒毛发育状况。结果显示Ⅰ组和Ⅱ组显著高Ⅲ组、Ⅳ组与Ⅴ组(P㩳0.05),Ⅰ组和Ⅱ组之间差异不显著(P㧐0.05),其中Ⅰ组小鼠的生长性能最好。在肠道形态学指标中,复合益生菌能显著提高小鼠十二指肠的绒毛长度。选取体重约1.2kg家兔75只,随机分为5组。I组(XOS+TPPPS+LP)、II组(XOS+LP)、III组(TPPPS+LP)、IV组(SA+SP)、Ⅴ组(PBS)。试验为期35d,在第0、7、14、21、28和35d对每组每只家兔进行空腹称重,计算每个阶段的平均日增重(ADG)和平均日采食量(ADFI),并计算饲料转化率(F/G);在7、14、21、28和35d每组随机选取3只家兔采血检测血清中总蛋白和胆固醇含量,剖杀后采集家兔肝脏和脾脏测定免疫器官指数,无菌采集盲肠内容物检测其乳酸杆菌和大肠杆菌的活菌数,采集十二指肠组织观察其形态并分析肠绒毛的发育状况。结果显示I组(XOS+TPPPS+LP)家兔的以上指标均有显著提高,其中在整个试验期Ⅰ组家兔的免疫器官指数和十二指肠形态学结果显著高于其他组(P㩳0.05),在生化性能的测定中I组家兔的指标显著高于对照组(P㩳0.05)。本研究表明,复合益生菌可以提高家兔的日增重和料肉比,提高机体的免疫力,有利于肠道益生菌的存活和繁殖,促进小肠绒毛的发育以及改善肠道内环境的稳态。
[Abstract]:The compound probiotics used in the production are mostly exogenous viable bacteria preparation, and the subsequent research finds that the oligosaccharide can selectively increase the proliferation of the intrinsic probiotics group in the intestinal tract of the animal, and is similar to the probiotic effect produced by the viable bacteria preparation. The functional oligosaccharide can not be directly digested and utilized in the gastrointestinal tract of the animal, but can be used by the probiotics of the intestinal tract, and has the effect of inhibiting the growth of the harmful bacteria, so that the functional oligosaccharide has an important influence on the microbial flora and the immune function of the gastrointestinal tract of the animal. The plant polysaccharide has rich nutrient components and active substances, and has the functions of resisting bacteria, resisting virus, resisting oxidation, improving the immune function of the animal body and the like, and is beneficial to the health of the body. In recent years, the development of China's animal husbandry has made remarkable achievements, but the problems of drug residue and other problems have also seriously affected the consumption of animal products and the export, research and development of mixed probiotics as one of the research directions of livestock and poultry industry in recent years. In the light of the previous studies, the study on the in vitro proliferation of L. plantarum by xylo-oligosaccharide and the polysaccharide of Taishan pine pollen showed that the number of colonies increased significantly after adding 20 mg/ mL of low polyxylose and 50 mg/ m L of Taishan Pollen in the bacterial liquid of L. plantarum. After the addition of 20 mg/ m L of xylo-oligosaccharide and 50 mg/ mL of Taishan Pollen, the growth of Lactobacillus plantarum in the medium with pH = 5 was good, the number of viable bacteria was higher than that of other groups, and the number of viable bacteria in the artificial simulated gastric juice was not affected, and the number of viable bacteria was reduced by about 10% after 3 h. And the number of viable bacteria in the artificial simulated intestinal fluid is unchanged. The results showed that Lactobacillus plantarum has the characteristics of probiotics, and the effect of addition of 20 mg/ mL of xylo-oligosaccharide and 50 mg/ mL of the polysaccharide of Taishan Pollen in the bacterial liquid is better, and the effect of its application in animal nutrition and daily food can be further studied. Seventy-five SPF Kunming mice with a weight of 25 g were randomly divided into 5 groups. Group I (xylo-oligosaccharide + Taishan pine pollen polysaccharide + Lactobacillus plantarum), Group II (xylo-oligosaccharide + Lactobacillus plantarum), Group 鈪，
本文编号：2481463
[Abstract]:The compound probiotics used in the production are mostly exogenous viable bacteria preparation, and the subsequent research finds that the oligosaccharide can selectively increase the proliferation of the intrinsic probiotics group in the intestinal tract of the animal, and is similar to the probiotic effect produced by the viable bacteria preparation. The functional oligosaccharide can not be directly digested and utilized in the gastrointestinal tract of the animal, but can be used by the probiotics of the intestinal tract, and has the effect of inhibiting the growth of the harmful bacteria, so that the functional oligosaccharide has an important influence on the microbial flora and the immune function of the gastrointestinal tract of the animal. The plant polysaccharide has rich nutrient components and active substances, and has the functions of resisting bacteria, resisting virus, resisting oxidation, improving the immune function of the animal body and the like, and is beneficial to the health of the body. In recent years, the development of China's animal husbandry has made remarkable achievements, but the problems of drug residue and other problems have also seriously affected the consumption of animal products and the export, research and development of mixed probiotics as one of the research directions of livestock and poultry industry in recent years. In the light of the previous studies, the study on the in vitro proliferation of L. plantarum by xylo-oligosaccharide and the polysaccharide of Taishan pine pollen showed that the number of colonies increased significantly after adding 20 mg/ mL of low polyxylose and 50 mg/ m L of Taishan Pollen in the bacterial liquid of L. plantarum. After the addition of 20 mg/ m L of xylo-oligosaccharide and 50 mg/ mL of Taishan Pollen, the growth of Lactobacillus plantarum in the medium with pH = 5 was good, the number of viable bacteria was higher than that of other groups, and the number of viable bacteria in the artificial simulated gastric juice was not affected, and the number of viable bacteria was reduced by about 10% after 3 h. And the number of viable bacteria in the artificial simulated intestinal fluid is unchanged. The results showed that Lactobacillus plantarum has the characteristics of probiotics, and the effect of addition of 20 mg/ mL of xylo-oligosaccharide and 50 mg/ mL of the polysaccharide of Taishan Pollen in the bacterial liquid is better, and the effect of its application in animal nutrition and daily food can be further studied. Seventy-five SPF Kunming mice with a weight of 25 g were randomly divided into 5 groups. Group I (xylo-oligosaccharide + Taishan pine pollen polysaccharide + Lactobacillus plantarum), Group II (xylo-oligosaccharide + Lactobacillus plantarum), Group 鈪，
本文编号：2481463
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