一株猪丹毒杆菌分离株的致病性与耐药性分析
发布时间：2019-05-23 05:04
【摘要】：从某猪场病死猪心血、肝、脾等组织分离到1株革兰阳性杆菌,经细菌形态、16S rRNA基因扩增,确定分离菌为猪丹毒杆菌。对分离菌进一步开展了药物敏感性试验、耐药基因的检测、细菌致病性试验及免疫保护性试验。结果发现:分离株对β-内酰胺类、大环内酯类、氟苯尼考高敏,但对氨基糖苷类、四环素、林可霉素、诺氟沙星等耐药;分离株可扩增出氨基糖苷类耐药基因aac C2和四环素耐药基因tetB,但菌株中没有扩增到四环素耐药基因tetA。细菌致病性试验表明该毒株对小鼠的LD50为1.77×10~5cfu/mL。小鼠免疫保护试验结果表明,疫苗G4T10免疫后28 d,以分离株攻毒,对照组全部死亡(5/5),免疫组小鼠攻毒后没有死亡,仅表现为体重减轻,表明现用猪丹毒疫苗能保护小鼠抵抗猪丹毒的致死性攻击。
[Abstract]:A strain of Gram-positive bacillus was isolated from the heart blood, liver, spleen and other tissues of dead pigs in a pig farm. After bacterial morphology and 16s rRNA gene amplification, the isolated bacteria were identified as Paeonia viridis. The drug sensitivity test, drug resistance gene detection, bacterial pathogenicity test and immune protection test were further carried out. The results showed that the isolates were resistant to 尾-lactam, macrolides, florfenicol Gao Min, but resistant to aminoglycosides, tetracycline, lincomycin and norfloxacin. The aminoglycoside resistance gene aac C2 and tetracycline resistant gene tetB, could be amplified by the isolate, but the tetracycline resistance gene tetA. was not amplified in the strain. The bacterial pathogenicity test showed that the LD50 of the strain to mice was 1.77 脳 10 ~ 5cfu / mL. The results of mouse immune protection test showed that 28 days after immunization with vaccine G4T10, all the mice in the control group died (5 鈮，
本文编号：2483628
[Abstract]:A strain of Gram-positive bacillus was isolated from the heart blood, liver, spleen and other tissues of dead pigs in a pig farm. After bacterial morphology and 16s rRNA gene amplification, the isolated bacteria were identified as Paeonia viridis. The drug sensitivity test, drug resistance gene detection, bacterial pathogenicity test and immune protection test were further carried out. The results showed that the isolates were resistant to 尾-lactam, macrolides, florfenicol Gao Min, but resistant to aminoglycosides, tetracycline, lincomycin and norfloxacin. The aminoglycoside resistance gene aac C2 and tetracycline resistant gene tetB, could be amplified by the isolate, but the tetracycline resistance gene tetA. was not amplified in the strain. The bacterial pathogenicity test showed that the LD50 of the strain to mice was 1.77 脳 10 ~ 5cfu / mL. The results of mouse immune protection test showed that 28 days after immunization with vaccine G4T10, all the mice in the control group died (5 鈮，
本文编号：2483628
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