亚麻籽和鱼油对蛋鸡蛋黄n-3多不饱和脂肪酸含量与肝脏脂肪酸代谢的影响

发布时间：2019-05-27 09:55

【摘要】：本试验旨在研究饲粮中添加亚麻籽和鱼油对蛋鸡蛋黄n-3多不饱和脂肪酸含量、肝脏脂肪酸组成及其合成代谢基因表达的影响。选择28周龄海兰褐壳蛋鸡96只,随机分为4组,每组24只。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加10%亚麻籽、10%亚麻籽+5%鱼油和5%鱼油。饲养21 d后连续收集7 d鸡蛋,然后每组随机选取4只鸡屠宰取样。结果表明,与对照组相比:1)添加亚麻籽和鱼油均显著增加蛋黄中n-3多不饱和脂肪酸含量(P0.05),其中单独添加鱼油的蛋黄中二十二碳六烯酸(DHA)和二十碳五烯酸含量最高;2)试验组肝脏单不饱和脂肪酸比例均显著降低(P0.05),肝脏n-3多不饱和脂肪酸比例均显著增加(P0.05);3)单独添加鱼油显著降低肝脏脂肪酸延长酶1、脂肪酸延长酶2和去饱和酶(脂肪酸去饱和酶1、脂肪酸去饱和酶2和硬脂酰辅酶A去饱和酶1)基因表达水平(P0.05)。由此可见,蛋鸡饲粮中单独添加亚麻籽或鱼油可以富集蛋黄n-3多不饱和脂肪酸沉积;添加亚麻籽促进肝脏α-亚麻酸转化生成DHA过程,表现为上调脂肪酸延长酶和去饱和酶基因表达,添加鱼油显示相反结果。
[Abstract]:The purpose of this study was to study the effects of flaxseed and fish oil on the content of polysaturated fatty acids in egg yolk, the composition of fatty acids in liver and the expression of synthetic metabolic genes in laying hens. 96 28-week-old Hailan brown shell laying hens were randomly divided into 4 groups with 24 hens in each group. The control group was fed with basic diet, and the experimental group was fed with 10% flaxseed, 10% flaxseed 5% fish oil and 5% fish oil, respectively. Eggs were collected for 7 days after 21 days of feeding, and then 4 chickens in each group were randomly selected for slaughtering and sampling. The results showed that compared with the control group, 1) adding flaxseed and fish oil significantly increased the content of n 鈮，

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